VARIOUS APPLICATIONS OF A METHOD OF A RESEARCH OF LEVELS OF CYTOKIN OF NEUTROPHILS



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Abstract

In a research approaches to assessment of levels of cytokin of neutrophils on the basis of secretion by neutrophils the preforming of cytokins after an hour incubation are offered the RPMI 1640 environment. Secretion of cytokins was defined with use of multiplex sets of the Biorad company (USA), Luminex (USA), for definition of various cytokins on the MAGPIX-100 device (USA) and the immunofermental analysis (IFA). It is established that at an incubation of neutrophils in the environment of RPMI 1640 within 1 hour in the environment of an incubation (supernatant) various cytokins come to light concentration of which in a supenatant of neutrophils exceeded reference level (RPMI-1640 environment). At addition to neutrophils of various bacteria, supernatant of bacteria, regulatory molecules, the activity of secretion changes (is stimulated or supressed) depending on that substrate which was added to the environment of an incubation of live neutrophils. Thus, the offered methodical approach of assessment of levels of cytokin of neutrophils allows to study mechanisms of regulation of cytokins network of these cages.

About the authors

A. V. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Author for correspondence.
Email: fake@neicon.ru

leader scientific researcher of the laboratory of immunology of infl ammation;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology,

Ekaterinburg

Russian Federation

V. A. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Email: fake@neicon.ru

senior scientific researcher of the laboratory of immunology of infl ammation;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology,

Ekaterinburg

Russian Federation

M. A. Dobrynina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

Junior scientific researcherof the laboratory of immunology of infl ammation,

Ekaterinburg

Russian Federation

L. O. Fomina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student,

Ekaterinburg

Russian Federation

A. I. Fayzullina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student,

Ekaterinburg

Russian Federation

V. A. Gritsenko

Orenburg Federal Research Сenter UrB RAS

Email: fake@neicon.ru

MD, professor, chief researcher,

Orenburg 

Russian Federation

References

  1. Долгушин И. И., Зурочка А. В., Чукичев А. В. Регуляторные пептиды нейтрофилов (нейтрофилокины). Иммунология. 1995, 4, 40–45.
  2. Зурочка А.В., Зурочка В. А., Зуева Е. Б., Добрынина М. А., Дукарт В. В., Черкасов С.В, Гриценко В. А. Влияние синтетического пептида активного центра гранулоцитарно-макрофагального колониестимулирующего фактора (ГМ-КСФ) на продукцию цитокинов нейтрофилами периферической крови человека. Бюллетень Оренбургского научного центра УрО РАН. 2015. 4: 1–9. [Электронный ресурс].
  3. Зурочка А. В., Хайдуков С. В., Кудрявцев И. В., Черешнев В. А. Проточная цитометрия в биомедицинских исследованиях. Екатеринбург: РИО УрО РАН, 2018, 720 с.
  4. Хайдуков С. В., Байдун Л. А., Зурочка А. В., Тотолян Арег. А. Стандартизованная технология «Исследование субпопуляционного состава лимфоцитов периферической крови с применением проточных цитофлюориметров-анализаторов». Российский иммунологический журнал. 2014. Т. 8 (17). 4: 974–992.
  5. Wong L., Wilson R. D. The indentification of Fc and C3 receptors on human neutrophils. J. Immunol. Meth. 1975. 7: 69–76.

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Copyright (c) 2019 Zurochka A.V., Zurochka V.A., Dobrynina M.A., Fomina L.O., Fayzullina A.I., Gritsenko V.A.

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