FEATURES OF THE REGULATORY INFLUENCES OF THE ARGINYL-ALPHA-ASPARTIL-LIZIL-VALYL-TYROSIL-ARGININ ON THE INTACT AND EXPERIMENTAL TRANSFORMED PHENOTYPE CD62L⁺CD63⁺CD66d⁺ OF NEUTROPHILIC GRANULOCYTES

The experimental model of the in vitro transformed phenotype of neutrophilic granulocytes (NG) equipped with receptors CD62L, CD63, CD66d, was created under the influence of N-formyl-methionyl-leucyl-phenylalanine (fMLP). The features of the transformed phenotype of NG were described. The influence of the hexapeptide arginyl-alpha-aspartyl-lysyl-poured-tyrosyl-arginine on untransformed and transformed phenotype of NG was investigated in vitro. It was shown that fMLP reduces the number of NG, expressing the receptor CD62L and reduces the density of expression of this receptor on the surface membrane of NG, moderately increases the density of CD63 and moderately increases the number of CD66dL⁺NG. Hexapeptide does not influence on the number of untransformed NG, bearing receptors CD62L, CD63, CD66d, and the level of the expression of those molecules. It has been shown the mitigation of the influences of fMLP after the simultaneous incubation NG with fMLP and hexapeptide: the number of CD62L⁺NG was increased and the density of membrane expression of the molecules of CD62L was increased too. At the same time, hexapeptide did not correct the negative effects of fMLP on the number of CD63⁺NG and CD66d⁺NG, and on level of density of the expression of CD63 and CD66d molecules on the surface membrane of NG. Overall, the results demonstrate the classic immunomodulatory effect of hexapeptide: on the one hand, we saw the absence of any changes of the studying receptors on membrane of the untransformed NG under hexapeptide influences, and on the other hand, it was demonstrated a strong trend of leveling the negative transformational effects of fMLP under hexapeptide influences.