Human placenta hydrolyzate affects neutrophils of systemic immunity during cosmetic procedures

In cosmetology, various types of procedures acting via varying mechanisms are used, including those based on rather aggressive mode of action. Few evidences have been collected so far regarding an effect of such procedures on human skin and entire body. Aim: to determine response of neutrophil arm of systemic immunity to injected placental hydrolyzate, compare effectiveness and safety of cosmetic methods affecting quality of skin layers. There were enrolled 75 healthy women subdivided into three groups with 25 subjects in each group. Patients in Group 1 underwent a course of intramuscular injections with human placental hydrolysate; Group 2 – fractional photothermolysis of facial skin layers with erbium laser; Group 3 – fractional photothermolysis procedure pretreated with course of intramuscular injected human placental hydrolyzate. Samples of peripheral blood were collected from all patients for assessing baseline state of the immune system before interventions and on day 8, 14 and 24 after the onset. It was shown that level of neutrophil phagocytosis and neutrophil NBT-assay were most markedly changed after interventions. It was found that level of neutrophil phagocytosis and neutrophil NBT-assay were virtually decreased by 2-fold after fractional photothermolysis, but not in patients pre-treated with injected placental hydrolysate followed by fractional photothermolysis. Neutrophil phagocytosis and spontaneous neutrophil NBT-assay data in treated vs. baseline patients did not change, whereas level of induced NBT-assay was decreased, but to much lower extent compared to control group solely treated with fractional photothermolysis. Conclusions: introduction of placental hydrolyzate contributes to accelerated rehabilitation period after applying aggressive medical interventions, and prevents development of adverse events.