Email this article VARIATION OF IMMUNE RESPONSE TO Tp0277, Tp0684, Тр0965 AND Тр1038 TREPONEMA PALLIDUM ANTIGENS IN DIFFERENT SYPHILIS STAGES
- Authors: Runina A.V.1, Zatevalov A.M.2, Katunin G.L.1, Deryabin D.G.1, Kubanov A.A.3
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Affiliations:
- State Scientific Centre of Dermatovenereology and Cosmetology, Russian Ministry of Health
- G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology
- Russian Medical Academy of Post-Graduate Education
- Issue: Vol 20, No 1 (2017)
- Pages: 70-78
- Section: ORIGINAL ARTICLES
- URL: https://rusimmun.ru/jour/article/view/94
- ID: 94
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Abstract
The level of IgG antibodies against recombinant proteins Tp0277, Tp0684, Тр0965, Тр1038 of T. pallidum was measured in ELISA assay using blood serum of patients with primary, secondary, early latent and late latent syphilis. We observed certain variations of ELISA diagnostic efficiency with above noted proteins depending on the form of the disease. 100% sensitivity in serodiagnostics of primary syphilis was observed in assays with Tp0277, secondary syphilis – with Tp0965, and early latent syphilis – with Tp1038 recombinant proteins. The highest antibody level against Tp0684 was observed in the group of late latent syphilis patients, but revealed a decreased sensitivity of the assay. The statistical discriminative analysis of the results enabled the development of classification rules for the differentiation of serum samples into four groups according to the stages of syphilis. The efficiency of the proposed classification model revealed 44.4–58.9%, and can be even higher up to 75% using pairwise comparison of sample groups. To summarize, this approach gives the opportunity for syphilis stage differentiation based on the analysis of antibodies against Treponema pallidum antigens panel.
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About the authors
A. V. Runina
State Scientific Centre of Dermatovenereology and Cosmetology, Russian Ministry of Health
Email: fake@neicon.ru
research fellow
Moscow
Russian FederationA. M. Zatevalov
G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology
Email: fake@neicon.ru
senior research fellow, PhD
Moscow
Russian FederationG. L. Katunin
State Scientific Centre of Dermatovenereology and Cosmetology, Russian Ministry of Health
Email: fake@neicon.ru
PhD, Head of Syphilidology Department
Moscow
Russian FederationD. G. Deryabin
State Scientific Centre of Dermatovenereology and Cosmetology, Russian Ministry of Health
Author for correspondence.
Email: dgderyabin@yandex.ru
M.D., Prof., Head of the Department of STD and Dermatoses Laboratory Diagnostics
107076 Moscow, Korolenko Str., 3/6
Russian FederationA. A. Kubanov
Russian Medical Academy of Post-Graduate Education
Email: fake@neicon.ru
M.D., Prof., corresponding member of RAS, Head of Dermatovenereology and Cosmetology Department
Moscow
Russian FederationReferences
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