Body sensitization to various antigens in children with chronic hepatitis B and concomitant giardiasis

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Abstract

The aim of the study was to evaluate body sensitization by measuring antigen-binding lymphocytes specific to various antigens in children with chronic hepatitis B and intestinal giardiasis.
The study of ASL was carried out in 126 children with CHB and 30 apparently healthy children aged 4 to 14 years. Among children with CHB, 93 had confirmed intestinal giardiasis (group I), and the remaining 33 were patients without lambliasis (group II). Sensitization and an autoimmune process were detected by assessing activity of specific immunity parameters such as ASL amount by indirect rosette formation against HBsAg, G. lamblia and intestinal tissue. We found that intensity of disease activity and ASL level against the examined antigens were paralleled evidencing about sensitization and autoimmune reactions related to giardiasis invasion in children with CHB. Depending on the duration of CHB with lamblial invasion ASL study data showed that prolonging disease duration resulted in lowered ASL to HBsAg peaking upon disease lasting up to 3 years. Apparently, it might be accounted for by body mobilization fighting against infection in general biological sense, and their further decline may occur due to host adaptation to increased antigenic load. The amount of ASL to the intestinal tissue was also somehow related with duration of the disease. The data obtained indicate increased self-sensitization along with increased duration of the disease. Similar data were obtained while studying amount of ASL to G. lamblia, thereby confirming a role of sensitization and self-sensitization in pathogenetic mechanisms of the developing CHB with giardial invasion especially during long-term course. Thus, the course of CHB with lambliasis invasion is accompanied by reaction of specific immune arm. Rise in amount of ASL to HBsAg, intestinal tissue and G. lamblia occurs along with deteriorating degree of disease activity and duration of hepatic disease suggesting about importance of sensitization and self-sensitization in pathogenesis of CHB development with giardiasis invasion, aggravating the course and outcome of the disease. The data reliability allows to conclude that giardiasis infection and prolongation of both hepatic pathological process and entire infectious process in children with CVH are directly interconnected. These data clearly underscore importance of timely eradication of G. lamblia upon liver damage. At the same time, administration of specific drugs (scheme) should be performed by taking into account its hepatotoxicity, bioavailability and effectiveness.

About the authors

N. F. Nurmatova

Tashkent Medical Academy

Author for correspondence.
Email: nargiza-nurmatova78@yandex.ru

Nurmatova Nargiza F. - PhD, MD (Medicine), Pediatrician-Allergist, Associate Professor, Department of Childhood Diseases No. 1

100109, Tashkent, Almazar district, City of doctors, 5, apt 7

Phone: +99890-321-34-78

Uzbekistan

N. Kh. Mirsalikhova

Tashkent Medical Academy

Email: fake@neicon.ru

PhD, MD (Medicine), Pediatrician, Associate Professor, Department of Childhood Diseases No. 1

Tashkent

Uzbekistan

M. А. Asilbekova

Tashkent Medical Academy

Email: fake@neicon.ru

PhD (Medicine), Pediatrician, Assistant Professor, Department of Childhood Diseases No. 1

Tashkent

Uzbekistan

F. Kh. Irsalieva

Tashkent Medical Academy

Email: fake@neicon.ru

PhD, MD (Medicine), Allergist-Immunologist, Associate Professor, Department of Allergology and Immunology

Tashkent

Uzbekistan

N. D. Dustbabaeva

Tashkent Institute for Post-Graduate Medical Education

Email: fake@neicon.ru

Assistant Course in Clinical Allergology, Allergologist-Immunologist

Tashkent

Uzbekistan

J. Kh. Akhmedov

LLC “Granat Stom”

Email: fake@neicon.ru

PhD (Medicine), Dentist

Nazarbek village, Tashkent region

Uzbekistan

References

  1. Ивашкин В.Т., Герман Е.Н., Маевская М.В. Скрытая инфекция вирусом гепатита В // Российский журнал гастроэнтерологии, гепатологии, колопроктологии, 2008. № 2. С. 4-11. [Ivashkin V.T., German E.N., Mayevskaya M.V. Hidden infection with hepatitis B virus. Rossiyskiy zhurnal gastroenterologii, gepatologii, koloproktologii = Russian Journal of Gastroenterology, Hepatology, Coloproctology, 2008, no. 2, pp. 4-11. (In Russ.)]
  2. Ивашкин В.Т., Буеверов А. Хронические заболевания печени сегодня и завтра // Врач, 2004. № 6. С. 4-6. [Ivashkin V.T., Bueverov A. Chronic liver disease today and tomorrow. Vrach = Doctor, 2004, no. 6, pp. 4-6. (In Russ.)]
  3. Корниенко Е.А., Минина С.Н., Фадина С.А., Калинина Н.М., Суворов А.Н. Диагностика и лечение лямблиоза у детей // Инфекционные болезни, 2009. Т. 7, № 1. C. 43-48. [Kornienko E.A., Minina S.N., Fadina S.A., Kalinina N.M., Suvorov A.N. Diagnosis and treatment of giardiasis in children. Infektsionnyye bolezni = Infectious diseases, 2009, Vol. 7, no. 1, pp. 43-48. (In Russ.)]
  4. Куропатенко М.В., Бандурина Т.Ю., Безушкина Н.А. Паразитозы, лямблиоз и аллергические заболевания в детском возрасте // Русский медицинский журнал, 2003. Т. 11, № 3. С. 143-146. [Kuropatenko M.V., Bandurina T.Yu., Bezushkina N.A. Parasitoses, giardiasis and allergic diseases in childhood. Russkiy meditsinskiy zhurnal = Russian Medical Journal, 2003, Vol. 11, no. 3, pp. 143-146. (In Russ.)]
  5. Caccio S.M., Ryan U. Molecular epidemiology of giardiasis. Mol. Biochem. Parasitol., 2008, Vol.160, no. 2, pp. 75-80.
  6. Heyworth M.F. Immunological aspects of Giardia infections. Parasite, 2014, Vol. 21, 55. doi: 10.1051/parasite/2014056.
  7. Ma’ayeh S.Y. Characterization of the Giardia intestinalis secretome during interaction with human intestinal epithelial cells: the impact on host cells. PLoS Negl. Trop. Dis., 2017, Vol. 11, no. 12, e0006120. doi: 10.1371/journal.pntd.0006120.
  8. Malik R., Hardikar W. Hepatitis B and C in Children. Indian J. Pediatr., 2016, Vol. 83, no. 11, pp. 1303-1310.
  9. Mysore K.R., Leung D.H. Hepatitis B and C. Clin. Liver Dis., 201, Vol. 22, no. 4, pp. 703-722.
  10. Shah U., Kelly D., Cahng M.H. et al. Management of chronic hepatitis B in children. J. Pediatr. Gastroenterol. Nutr., 2009, Vol. 48, no. 4, pp. 399-404.

Copyright (c) 2020 Nurmatova N.F., Mirsalikhova N.K., Asilbekova M.А., Irsalieva F.K., Dustbabaeva N.D., Akhmedov J.K.

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This work is licensed under a Creative Commons Attribution 4.0 International License.
Свидетельство о регистрации СМИ ПИ № 77 - 11525 от 04.01.2002 выдано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).


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