Email this article Effect of synthetic analogue of ZP2 peptide, an active center of GM-CSF, upon antilysozyme activity of Candida
- Authors: Pashinina O.A.1, Kartashova O.L.1, Pashkova T.M.1, Gritsenko V.A.1
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Affiliations:
- Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences, Branch of Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
- Issue: Vol 26, No 3 (2023)
- Pages: 373-376
- Section: SHORT COMMUNICATIONS
- URL: https://rusimmun.ru/jour/article/view/9645
- DOI: https://doi.org/10.46235/1028-7221-9645-EOS
- ID: 9645
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Abstract
Our aim was to analyze the effects of the ZP2 peptide, a synthetic analogue of active center of GM-CSF, upon antilysozyme activity (ALA) of Candida.
32 vaginal isolates of Candida spp were used in the work. Five species have been isolated from the vaginal secretions of the conditionally healthy pregnant women taken within a screening program. To study the effect of ZP2 on the ALA of the Candida fungi, the fungal cells were co-cultured with a ZP2 solution in Saburo broth medium at 37 oC for 24 hours. Thereafter, ALA of fungi was determined by photometric method.
It was found that the ZP2 peptide caused a decreased expression of ALA of vaginal Candida spp. Isolates, i.e., loss of ALA in the isolates of C. glabrata, C. tropicalis, C. krusei and some isolates of C. albicans, as well as a decreased level of ALA in C. kefir and other C. albicans isolates. Thus, the synthetic analogue of ZP2 peptide (active center of GM-CSF) showed an inhibitory effect upon the antilysozyme activity of Candida.
Full Text
Anti–lysozyme activity (ALA) is one of the persistence factors that ensure the tolerance of microorganisms to the action of human and animal lysozyme.
In yeast-like Candida ALA occurs in almost 100% of cases [3, 8]. The prevalence and severity of this trait were studied in fungi isolated from various diseases and from various biotopes of the human body, while it was revealed that the presence of fungi with a high level of ALA contributes to the long course of the infectious process and can be a criterion for its chronization [9, 10, 12, 14]. A number of authors have shown the influence of factors of various genesis on the severity of ALA in Candida [15, 16, 17]. However, the effect of the synthetic analogue of the active center of granulocyte-macrophage colony-stimulating factor (GM–CSF) - peptide ZP2 on the antilysozyme activity of Candida has not been previously studied.
At the same time, it is known that this peptide ZP2 has a wide spectrum of biological action [5, 7]. In particular, its modifying effects on the ability of S. aureus to form biofilms have been established [4], and its effect on the anti-cytokine activity of a number of microorganisms and their ability to produce cytokine-like substances has also been shown [6, 11].
The above points to the relevance of studying the effect of synthetic peptide ZP2 on the biological properties of Candida, including their anti-lysozyme activity and other persistence factors.
The aim is to analyze the nature of the effect of the synthetic analogue of the active center of GM–CSF - peptide ZP2 on the anti–lysozyme activity of Candida.
Materials and methods. 32 vaginal isolates of Candida spp were used in the work. five species (C. albicans, C. glabrata, C. tropicalis, C. krusei, C.kefir) isolated from the vaginal discharge from conditionally healthy pregnant women within the framework of regulated screening. Fungi were isolated according to [13], species identification of fungal isolates was carried out by a generally accepted method based on morphological and biochemical properties.
To study the effect of ZP2 on the ALA of Candida, 1 ml of mushroom suspension was cultured in saline solution with 1 ml of ZP2 solution with a concentration of 0.1 micrograms/ ml in 2 ml of Saburo broth at 37oC for 24 hours. Samples without the addition of ZP2 solution served as control. Then the control and experimental samples were centrifuged for 15 minutes at 3000 rpm, the filler liquid was drained, 2 ml of Saburo broth was added to the sediment and resuspended. Next, 150 µl of suspension was taken to assess the level of fungal ALA, which was determined by photometric method [2]. The data obtained were processed by methods of variational statistics [1].
Results.
It was found that representatives of all studied species of Candida showed ALA, but with different frequency. 100% of C. glabrata and C. krusei isolates, 86% – C. albicans, 75% – C. tropicalis and 50% – C. kefir were able to inactivate lysozyme.
The maximum level of ALA was observed in C. kefir (0.52±0.04 mcg/ml), the minimum – in C. tropicalis (0.18±0.01 mcg/ml), in C. albicans, C. glabrata and C. krusei, the severity of ALA was 0.39±0.01, 0.33±0.01 and 0.24±0.02 mcg/ml, respectively.
As a result of in vitro experiments, it was found that the synthetic peptide ZP2 caused loss of the ability to inactivate lysozyme in C. glabrata, C. tropicalis and C. krusei isolates, as well as in 50% of C. albicans strains. The peptide had no effect on the prevalence of the trait in C. kefir.
When analyzing the effect of synthetic peptide ZP2 on the severity of the antilysozyme trait in vaginal isolates of Candida spp. a significant decrease in the level of ALA was found in fungi of all studied species. Thus, in C. albicans, there was a 1.5–fold decrease in the severity of ALA from 0.39±0.01 to 0.27±0.03 micrograms/ml, and in C. kefir - 2.1-fold from 0.52±0.04 to 0.24±0.02 micrograms/ml (p<0.01) (Table 1).
Table 1
The effect of peptide ZP2 on the antilysozyme activity (АLА) of Candida spp.
| Prevalence, % | Expression, mcg/ml | ||
Control
|
An experience |
Control
|
An experience | |
C. albicans (n=16) | 86 | 43 | 0,39±0,01 | 0,27±0,03* |
C. glabrata (n=4) | 100 | 0 | 0,33±0,02 | 0* |
C. tropicalis (n=4) | 75 | 0 | 0,18±0,01 | 0* |
C. krusei (n=4) | 100 | 0 | 0,24±0,01 | 0* |
C. kefir (n=4) | 50 | 50 | 0,52±0,04 | 0,24±0,02* |
Explanation: * - p<0.01. / Explanation: * - p<0.01
Conclusion. The effect of the synthetic analogue of the active center of granulocyte-macrophage colony-stimulating factor (GM–CSF) - peptide ZP2 on the antilysocyme activity of strains of Candida isolated from the reproductive tract of conditionally healthy pregnant women was characterized for the first time. It was found that the peptide ZP2 had a modifying effect on the anti-lysozyme activity of Candida, reducing both the prevalence and severity of ALA.
The obtained materials open up the possibility of using the ZP2 peptide as a substance that can be effective in the treatment of infections caused by persistent strains of Candida, as well as for the correction of vaginal microflora when fungi of this genus are carried.
About the authors
Olga A. Pashinina
Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences, Branch of Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Author for correspondence.
Email: olga25mikro@mail.ru
PhD (Biology), Senior Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, OrenburgOlga L. Kartashova
Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences, Branch of Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: labpersist@mail.ru
PhD, MD (Biology), Associate Professor, Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, OrenburgTatiana M. Pashkova
Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences, Branch of Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: labpersist@mail.ru
PhD, MD (Biology), Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, OrenburgViktor A. Gritsenko
Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences, Branch of Orenburg Federal Research Center, Ural Branch, Russian Academy of Sciences
Email: vag59@mail.ru
PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, OrenburgReferences
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