CONTENT OF CHEMOKINES RANTES, IL-8 AND MIP-1B IN PLATELET LYSATES OBTAINED FROM DONOR BLOOD



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Abstract

Abstract

Growth supplements obtained from animal serum have a rather labile range of a specific growth factor content and can potentially be contaminated. On the other hand, growth supplements obtained from animal raw materials contain a composition of proteins, vitamins and microelements necessary for full-fledged cell growth, and these disadvantages can be overcome by replacing them with a product obtained from human donor blood. Platelet lysate contains biologically active molecules contained in various granules of these formed elements of the blood. The concentration of individual components in the resulting product varies and apparently depends not only on gender and blood group, but also on other, as yet unidentified individual characteristics. The aim of the study was to examine the content of some members of the chemokine family, in particular: RANTES, MIP-1b and IL-8 in platelet lysates obtained from healthy donors. The highest concentrations of the cytokine MIP-1b were noted in donors with Rh-negative affiliation, in women with the third and men with the fourth blood group. The highest values ​​of IL-8 content in platelet lysate were also recorded in men and women with Rh-negative blood of the fourth group. Platelet lysate of volunteers with the second blood group (men Rh-negative, women Rh-positive) contained the highest amount of RANTES. Reliable differences were found between the level of the MIP-1b factor in the thrombolysate of men and women with the second A (II) and fourth AB (IⅤ) Rh-negative blood groups; between women of the third B (III) blood group with different Rh factors. In addition, statistically significant differences were found in the RANTES/CCL5 chemokine values ​​in the blood thrombolysate in the groups of men and women with the second A (II) (with R (+) and R (-)) and fourth AB (IⅤ) blood groups (with R (+)). Interestingly, in men with the first 0 (I) and second A (II) blood groups with a negative Rh factor, the content of the chemokine RANTES/CCL5 in the blood thrombolysate significantly exceeded similar values ​​in the blood obtained from Rh-positive men. The relationship between the donors' belonging to a particular blood group and the content of these chemokines is not yet clear; in the future, deep bioinformatics analysis will reveal the as yet unclear communication between these indicators.

About the authors

Valeria Vladimirovna Shupletsova

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: vshupletsova@mail.ru
ORCID iD: 0000-0001-7243-9731
SPIN-code: 5736-4492
Scopus Author ID: 56440201700

Candidate of Biological Sciences, Senior Researcher, Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Olga Borisovna Melashchenko

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: OMelashchenko@kantiana.ru
ORCID iD: 0000-0002-0892-1168
SPIN-code: 2600-9368
Scopus Author ID: 22234973300

Researcher, Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Natalya Dinislamovna Gazatova

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: n_gazatova@mail.ru
ORCID iD: 0000-0002-4646-3436
SPIN-code: 1174-7330
Scopus Author ID: 57189342795

Candidate of Biological Sciences, Head of the Laboratory of Experimental Blood Preparations, Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Olga Genadyevna Khaziakhmatova

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: hazik36@mail.ru
ORCID iD: 0000-0002-5525-3529
SPIN-code: 5906-9527
Scopus Author ID: 55820782400

Candidate of Biological Sciences, Senior Researcher, Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Alexandra Sergeevna Minina

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: minina.saschenka@yandex.ru
ORCID iD: 0009-0000-2222-2649

Research Student, Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Anastassiya Mikhailovna Kopteva

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: vohumanaah@gmail.com
ORCID iD: 0009-0002-4722-643X

Research Student, Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Nina Avgustovna Kabanchuk

GBUZ "Blood Transfusion Station of the Kaliningrad Region", Ministry of Health of the Kaliningrad Region, Kaliningrad, Russia

Email: spk@infomed39.ru
ORCID iD: 0009-0004-9655-4223

Acting Chief Physician, Transfusiologist, Kaliningrad Region Blood Transfusion Station

Russian Federation, 29 Chkalova str., Kaliningrad, 236022, Russia

Tatyana Nikolaevna Pervenetskaya

GBUZ "Blood Transfusion Station of the Kaliningrad Region", Ministry of Health of the Kaliningrad Region, Kaliningrad, Russia

Email: pervenetskaia.tatiana@gmail.com
ORCID iD: 0009-0006-0073-5455

Transfusiologist, Head of the expedition with the Center for inventory management of donated blood components

Russian Federation, 29 Chkalova str., Kaliningrad, 236022, Russia

Andrey Gennadievich Goncharov

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Email: agoncharov59@mail.ru
ORCID iD: 0000-0001-6967-8838
SPIN-code: 7159-0402
Scopus Author ID: 57196504924
ResearcherId: D-3170-2018

Candidate of Medical Sciences, Senior Researcher, I. Kant BFU Center for Immunology and Cellular Biotechnologies

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

Larisa Sergeevna Litvinova

Federal State Autonomous Educational Institution of Higher Education "Immanuel Kant Baltic Federal University" of the Ministry of Science and Higher Education of the Russian Federation, Kaliningrad, Russia

Author for correspondence.
Email: larisalitvinova@yandex.ru
ORCID iD: 0000-0001-5231-6910
SPIN-code: 6703-3412
Scopus Author ID: 7007068521

Doctor of Medical Sciences, Director of the Center for Immunology and Cellular Biotechnology

Russian Federation, Alexander Nevsky St., 14, Kaliningrad, Russia, 236041

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Copyright (c) Goncharov A.G., Shupletsova V.V., Melashchenko O.B., Gazatova N.D., Khaziakhmatova O.G., Minina A.S., Kopteva A., Kabanchuk N.A., Pervenetskaya T.N., Litvinova L.S.

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