ALTERATION OF IFN-α AND IFN-β GENE EXPRESSION BY siRNA TARGETING THE Nup98 GENE DURING HSV-1 INFECTION IN VITRO



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Abstract

Abstract

HSV-1 infections are extremely common in the human population. A wide range of drug therapy and other approaches are currently used to treat herpes infection, but over time, an increase in the incidence of HSV-1 infection in the population is observed. A promising therapeutic approach in this situation may be the use of RNA interference. During its reproduction, HSV-1 imports viral DNA through the nuclear pore complex (NPC), located in the membrane of the cell nucleus and consisting of nucleoporin proteins (Nup98, Nup205, NXF1 and others). Therefore, disruption of the NPC structure as a result of siRNA-mediated inhibition of nucleoporin protein formation may hypothetically lead to a decrease in HSV-1 reproduction. The aim of this study is to evaluate the antiviral effect of siRNAs targeting the cellular gene Nup98, as well as to assess the dynamics of expression of cytokines IFN-α and IFN-β in an in vitro model.

About the authors

Evgeny Alekseevich Pashkov

FGBNU “Scientific Institution I. I.Mechnikov Research Institute of Vaccines and Serums " Ministry of Education and Science of Rus;
FGAOU VO "First Moscow State Medical University named after I.M. Sechenov” of the Ministry of Health of Russia (Sechenov University)

Email: pashckov.j@yandex.ru
ORCID iD: 0000-0002-5682-4581

Junior Researcher, Laboratory of Molecular Immunology, Federal State Budgetary Scientific Institution I. I. MechnikovResearch Institute of Vaccines and Serums; assistant of the department of microbiology, virology and immunology Sechenov University

Russian Federation

Liana Alexandrovna Kulikova

FGBNU “Scientific Institution I. I.Mechnikov Research Institute of Vaccines and Serums " Ministry of Education and Science of Russia;
FGAOU VO "First Moscow State Medical University named after I.M. Sechenov” of the Ministry of Health of Russia (Sechenov University)

Email: LianaKulikovaMira@yandex.ru
ORCID iD: 0009-0005-0203-1382

3rd year student of the I.M. Sechenov First Moscow State Medical University; laboratory assistant researcher of the laboratory of molecular immunology of the I.I. Mechnikov Research Institute of Vaccines and Sera

Russian Federation

Oxana Anatolevna Svitich

FGBNU “Scientific Institution I. I.Mechnikov Research Institute of Vaccines and Serums " Ministry of Education and Science of Russia;
FGAOU VO "First Moscow State Medical University named after I.M. Sechenov” of the Ministry of Health of Russia (Sechenov University)

Email: svitichoa@yandex.ru
ORCID iD: 0000-0003-1757-8389
SPIN-code: 8802-5569

Corresponding member of RAS, MD, The Head of I.I. Mechnikov Research Institute of Vaccines and Sera, Professor of Microbiology, Virology and Immunology department of the I.M. Sechenov First Moscow State Medical University

Russian Federation

Vytaly Vasilevich Zverev

FGBNU “Scientific Institution I. I.Mechnikov Research Institute of Vaccines and Serums " Ministry of Education and Science of Russia;
FGAOU VO "First Moscow State Medical University named after I.M. Sechenov” of the Ministry of Health of Russia (Sechenov University)

Author for correspondence.
Email: vitalyzverev@outlook.com
ORCID iD: 0000-0002-0017-1892
SPIN-code: 2122-1808

Academician of RAS, Doctor of Biological Sciences, Scientific Adviser of I.I. Mechnikov Research Institute of Vaccines and Sera, Professor, The Head of Microbiology, Virology and Immunology department of the I.M. Sechenov First Moscow State Medical University

Russian Federation

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Copyright (c) Pashkov E.A., Kulikova L.A., Svitich O.A., Zverev V.V.

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