DETECTION OF CYTOKINE-LIKE SUBSTANCES WITH THE HELP OF A TEST SYSTEM FOR THE DETERMINATION OF CYTOKINES IN A MOUSE IN STAPHYLOCOCCUS AUREUS, OPPOSITE FOR SPA GENE

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Abstract

In the work, cytokine-like activity was studied in 6 strains of Staphylococcus aureus, with and without genetic determinants of staphylococcal protein A (spa gene). The spa gene in S. aureus was determined by the polymerase chain reaction (PCR) method. The production of cytokine-like substances in the supernatants of S. aureus daily broth cultures was determined using an 8-plex immunofl uorescence test system for the determination of mouse cytokines from BIO-RAD (USA) (GM-CSF, INF-γ, IL-1b, IL-2, IL-4, IL-5, IL-10, TNF-α) on the device MAGPIX-100 (USA). It was established that, regardless of the presence or absence of genetic markers of staphylococcal protein A, cytokine-like substances were detected in the supernatants of the studied strains of S. aureus. Thus, the cytokine-like activity of S. aureus, detected using a multiplex mouse test cytokine test system, does not depend on the presence of the spa gene in bacteria, which is responsible for the production of staphylococcal protein A.

About the authors

A. V. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Author for correspondence.
Email: av_zurochka@mail.ru

leader scientific researcherof the laboratory of immunology of inflammation, Ekaterinburg;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology, Chelyabinsk

Russian Federation

V. A. Zurochka

Institute of Immunology and Physiology UrB RAS;
South-Ural State University (National Research University)

Email: fake@neicon.ru

senior scientific researcherof the laboratory of immunology of inflammation, Ekaterinburg;

MD, professor of the Department of food and biotechnology, senior researcher of the laboratory of molecular immunology, Chelyabinsk

Russian Federation

L. O. Fomina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student,

Ekaterinburg

Russian Federation

A. I. Fayzullina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

post-graduate student,

Ekaterinburg

Russian Federation

M. A. Dobrynina

Institute of Immunology and Physiology UrB RAS

Email: fake@neicon.ru

Junior scientific researcherof the laboratory of immunology of inflammation,

Ekaterinburg

Russian Federation

V. A. Gritsenko

Orenburg Federal Research Сenter UrB RAS

Email: fake@neicon.ru

MD, Professor, Chief Researcher,

Orenburg

Russian Federation

References

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  3. Зурочка А. В., Дукардт В. В., Зурочка В. А., Добрынина М. А., Зуева Е. Б., Тяпаева Я. В., Гриценко В. А. Стафилококки как продуценты цитокиноподобных веществ. Российский иммунологический журнал. 2017, 11 (20), 2, 134–136.
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  5. Гриценко В. А., Мавзютов А. Р., Пашкова Т. М., Карташова О. Л., Тяпаева Я. В., Белозерцева Ю. П. Генетический профиль Staphylococcus aureus, выделенных от бактерионосителей и больных с инфек ци он но-воспалительной патологией. Журн. микробиол., эпидемиол. и иммунобиол. 2018, 4, 56–62.
  6. Sabat A., Krzyszton-Russjan J., Strzalka W., Filipek R., Kosowska K., Hryniewicz W., Travis J., Potempa J. Newmethod for typing Staphylococcus aureus strains: multiple-locus variable-number tandem repeat analysis of polymorphism and genetic relationships of clinical isolates. J. Clin. Microbiol. 2003. 41(4): 1801–1804.
  7. Фомина Л. О., Файзуллина А. И., Зурочка В. А., Добрынина М. А., Симбирцев А. С., Гриценко В. А. Сравнительная оценка цитокиноподобной активности Staphylococcus aureus мультиплексным и иммуноферментным анализом. Российский иммунологический журнал. 2018, 12 (21), 3, 460– 465.

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Copyright (c) 2019 Zurochka A.V., Zurochka V.A., Fomina L.O., Fayzullina A.I., Dobrynina M.A., Gritsenko V.A.

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