A STUDY ON THE ASSOCIATION OF IL‑1RAVNTR AND IL‑1α (С+889T) GENE POLYMORPHISMS WITH FEATURES OF ESSENTIAL HYPERTENSION

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Abstract

Objective: the analysis of the relationship between polymorphisms IL1-raVNTR, С+889T gene IL-1α and features of essential hypertension (the risk myocardial infarction and stroke among men and women).

Methods: The 300 patients with II stage essential hypertension were followed for 8 years. Polymorphism of cytokine genes (IL-1ra (86 bp VNTR),–889C/T IL-1α) were examined by the method restrictor analysis of amplification products specific regions of the genome. Concentrations of IL-1ra, IL-1α in serum were measured by ELISA (test systems eBioscience (Bender MedSystems)).

Results: Genotyping of interleukin 1 receptor antagonist genes (IL-1ra86bp VNTR) has shown positive associations between the intron 2 IL-1ra – 4R/4R, 5R/5R microsatellite polymorphism and the risk of arterial hypertension development. The 5R/5R, 2R/2R were associated significantly with risk of stroke and myocardial infarction in patients with essential hypertension. Patients with polymorphisms of IL-1ra 4R/4R and 5R/5R have a lower concentration of IL-1Pa and higher levels of IL-1α in serum than otherpatients. Variant TT polymorphism –889C/T IL-1α is correlated with an increase in the concentration of IL-1α in the serum, without changing the frequency of hypertension, but increases the risk of myocardial infarction and stroke in patients with hypertension (mostly women). Allele 4R/4R (IL1-raVNTR) is associated with hypertension and its combination with – TT variant with IL-1alpha (C + 889T) polymorphisms contribute to the development of myocardial infarction and stroke in women.

About the authors

O. A. Radaeva

Mordovian State N. P. Ogariov University

Author for correspondence.
Email: vtlbwbyf_79@mail.ru

PhD (Medicine), docent of chair of Immunology, Microbiology, Virology

26a, St. Ulyanova, Saransk

Russian Federation

A. S. Simbirtsev

State Research Institute of Highly Pure Biopreparations

Email: fake@neicon.ru

PhD, MD (Medicine), Professor, Corresponding member of the Russian Academy of Sciences, deputy director of Institute of Highly Pure Biopreparations

7, St. Pudozhsky, St. Petersburg

Russian Federation

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