Email this article MOLECULAR CLONING OF HUMAN THYROID STIMULATING HORMONE RECEPTOR GENE
- Authors: Zubkov A.V.1, Andreeva M.A.1, Sidorov A.V.1, Milovanova A.V.1, Butova L.G.1
-
Affiliations:
- Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
- Issue: Vol 22, No 2-1 (2019)
- Pages: 278-280
- Section: ORIGINAL ARTICLES
- Submitted: 17.04.2020
- Accepted: 17.04.2020
- Published: 15.04.2019
- URL: https://rusimmun.ru/jour/article/view/121
- DOI: https://doi.org/10.31857/S102872210006600-5
- ID: 121
Cite item
Full Text
Abstract
Producing of recombinant TSHR fragments opens up new possibilities in the development of immunochemical tests to detect stimulating and blocking antibodies to TSHR, in studying the role of the antigenic structure of TSHR in the pathogenesis of autoimmune diseases of the thyroid gland. The original mRNA sequence of the α-subunit of the thyroid stimulating hormone receptor, not containing nucleotide substitutions, was cloned. The obtained recombinant vector pVAX1-TSHR, prepared for transfection into cells of mammals and for the expression of the recombinant protein of the α-subunit of the thyroid stimulating hormone receptor.
Keywords
About the authors
A. V. Zubkov
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Author for correspondence.
Email: alex_zubkov@list.ru
M. D. PhD, Head of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
Russian FederationM. A. Andreeva
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
junior researcher, of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
Russian FederationA. V. Sidorov
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
Ph. D. Head of the Laboratory for Genetics of DNA viruses,
Moscow
Russian FederationA. V. Milovanova
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
researcher of the Laboratory for Genetics of DNA viruses,
Moscow
Russian FederationL. G. Butova
Federal State Budgetary Scientific Institution «I. Mechnikov Research Institute of Vaccines and Sera»
Email: fake@neicon.ru
Ph.D., leading researcher, of the Laboratory for Immunological Diagnostics of Endocrine Diseases,
Moscow
Russian FederationReferences
- Smith T. J. TSHR as a therapeutic target in Graves’ disease. Expert Opin Ther Targets. 2017; 21(4): 427– 432.
- Rapoport B., McLachlan S. M. TSH receptor cleavage into subunits and shedding of the A-subunit; a molecular and clinical perspective. Endocrine Reviews. 2016; 37(2): 114–34.
- Sanders J., Bolton J., Sanders P., Jeff reys J., Nakatake N., Richards T., Evans M., Kiddie A., Summerhayes S., Roberts E., Miguel R. N., Furmaniak J., Smith B. R. Eff ects of TSH receptor mutations on binding and biological activity of monoclonal antibodies and TSH. Thyroid. 2007; 17(2): 1195–1206.
- Furmaniak J., Sanders J., Núñez Miguel R., B. Rees Smith. Mechanisms of Action of TSHR Autoantibodies. Horm Metab Res. 2015; 47: 735–752.
- Parmentier M., Libert F., Maenhaut C., Lefort A., Gerard C., Perret J., Van Sande J., Dumont J. E., Vassart G. Molecular cloning of the thyrotropin receptor. Science. 1989; 246(4937): 1620–1622.
Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).