SPONTANEOUS PRODUCTION OF CYTOKINES IN THE SUPERNATANTS OF CELLS ISOLATED FROM PERIPHERAL BLOOD, LYMPH NODES, SPLEEN, THYMUS AND SKIN IN A MODEL OF ACUTE PSORIASIS-LIKE DERMATITIS IN MICE

Imiquimod-induced model of psoriasis in mice are used for the study of psoriasis. Imiquimod in the form of skin applications  triggers a complex multi-stage process simulating psoriatic inflammation of the skin, accompanied by a change in cytokine production. The aim of this work was a comprehensive study of the production of cell supernatants isolated from skin, blood, from central and peripheral lymphoid organs and the effect of imiquimod on it. 46 mice of the C57BL/6 line were divided into two groups - control, n-22 and experimental, n-24. The formation of experimental pathology was carried out by the method of L. van der Fits. Mice of the experimental group were given a cream with 5% imiquimod (62.5 mg/cm2/day/mouse) for 7 days. On the 7th day of the experiment, blood samples were taken, the spleen, lymph nodes, thymus were extracted, and skin biopsy was obtained. To obtain a cell suspension, biopsies of the spleen, lymph nodes, and thymus were crushed in a homogenizer, and then passed through cell fittings. The method of spontaneous migration was used to isolate cells from the skin. The culture of blood, spleen, lymph nodes, thymus, and skin cells was incubated for 24 hours in RPMI-1640, then spontaneous cytokine production was investigated. The cytokine level (INF-γ, IL-1β, IL-5, IL-6, IL-10, IL-17, TNF-α, IL-10) was determined using the Mouse Th1/Th2/Th17 Panel test system. The study revealed that cells isolated from blood and lymphoid organs synthesize increased amounts of pro-inflammatory cytokines: IL-1 and IL-17. The most pronounced changes in the cytokine profile are observed in the blood, skin and spleen supernatants.