ACTIVITY OF CELLULAR IMMUNITY IN PATIENTS WITH NUTRITIONAL-DEPENDENT DISEASES (OBESITY AND TYPE 2 DIABETES)



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Abstract

Abstract

The purpose of the study is to analyze the indicators of cellular immunity in patients with obesity (OB) and type 2 diabetes mellitus (DM2). The study included 15 patients with DM2 (DM2 group), body mass index (BMI) 37.36±1.12 kg/m2, 12 patients with OB (OB group), BMI - 38.12±1.51 kg/m2 and 15 practically healthy individuals (comparison group - GС): BMI 23.5±0.15 kg/m2. The study was performed on a flow cytometer FC-500 (Beckman Coulter, USA) using double combinations of monoclonal antibodies (Beckman Coulter - Immunotech SAS, France). The percentage indicators of the T-cell population were assessed [total number of T-lymphocytes (CD3+), number of T-helper cells (CD3+CD4+), cytotoxic T-lymphocytes (CD3+CD8+), natural killer cells - NK cells (CD3- CD16+CD56+), natural killer cells with the properties of T lymphocytes - NKT cells (CD3+CD16+CD56+) and B-cell population (CD19+) lymphocytes, as well as the relative content of lymphocytes carrying activation markers (CD3+HLA- DR+, CD3+CD25+), and apoptosis marker antigen CD45+CD95+. The relative content of B-lymphocytes and T-lymphocytes in the peripheral blood of the examined individuals did not have statistically significant differences. In patients in the DM2 and OB groups, there was a tendency (p<0.1) towards an increase in the relative content of T-helper cells and a significant (p<0.05) decrease in the percentage of T-cytotoxic lymphocytes relative to GC. This redistribution of lymphocyte subpopulations led to a significant (p<0.05) increase in the IRI value (arbitrary units): DM2 – 2.87±0.58; OB – 2.30±0.33 vs GC – 1.62±0.15. The relative content of NK cells and NKT cells in the peripheral blood of the examined individuals did not have a statistically significant difference in the magnitude of the indicators.. A statistically significant (p <0.05) increase in the relative content of T-lymphocytes expressing the activation marker HLA-DR (CD3+ HLA-DR+) was found in patients in the DM2 and OB groups relative to GC [(%) DM2 – 7.95±0, 81; OB – 6.54±0.24; GC - 4.01±0.91] and a significant (p<0.05) increase in the percentage of CD45+CD95+ lymphocytes in patients with OB and DM2 relative to GC [(%) DM2 - 5.84±0.68; OB – 5.16±0.89; GC - 2.78±0.34]. The results obtained indicate the presence of meta-inflammation in patients with DM2 and OB.

About the authors

Eleonora Nikolaevich Trushina

Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia

Email: trushina@ion.ru
ORCID iD: 0000-0001-7149-2485
SPIN-code: 9908-6436
Scopus Author ID: 6603931370
ResearcherId: J-7366
http://orcid.org/0000-0001-7149-2485

PhD, Head of the Laboratory of Immunology, Federal Research Centre of Nutrition, Biotechnology and Food Safety

Russian Federation, 2/214 Ustynsky proezd, Moscow

Oksana Konstantinovna Mustafina

Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia

Email: mustafina@ion.ru
ORCID iD: 0000-0001-7231-9377
SPIN-code: 6201-5422
Scopus Author ID: 7003651519
ResearcherId: J-7258
http://orcid.org/0000-0001-7231-9377

PhD, Senior Researcher, Laboratory of Immunology, Federal Research Centre of Nutrition, Biotechnology and Food Safety

Russian Federation, 109240 Ustinsky proezd, 2/14, Moscow

Khaider Hamzyarovich Sharafetdinov

Federal Research Centre of Nutrition, Biotechnology and Food Safety, Moscow, Russia;
Russian Medical Academy of Continuing Professional Education of the Ministry of Health of the Russian Federation, Moscow, Russia;
First Moscow State Medical University named after I.M. Sechenov" of the Ministry of Health of the Russian Federation (Sechenov University), Moscow, Russia

Author for correspondence.
Email: sharafandr@mail.ru
ORCID iD: 0000-0001-6061-0095
SPIN-code: 8612-0792
Scopus Author ID: 6603694851
ResearcherId: R-9701-2016
http://orcid.org/0000-0001-6061-0095

MD, Head of the Department of Metabolic Diseases and diet therapy of the Clinical Nutrition Clinic Federal Research Centre of Nutrition, Biotechnology and Food Safety

Russian Federation, 109240, Moscow, Ustinsky proezd, 2/14

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