IDENTIFICATION OF IgE - MEDIATED SENSITIZATION IN CHILDREN BY ENZYME IMMUNOASSAY AND IMMUNOFLUORESCENCE METHODS
- Authors: Shcheglova E.L.1, Sokolova T.F.1, Sokolov E.Y.2, Emelyanov Y.V.3
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Affiliations:
- Omsk State Medical University, Omsk, Russia
- Scientific and Clinical Center «Karpovka», St.Peterburg, Russia
- Pirogov Russian National Research Medical University, Institute of Clinical Psychology and Social Work, Moskou, Russia
- Section: Joint Immunology Forum 2024
- URL: https://rusimmun.ru/jour/article/view/16710
- DOI: https://doi.org/10.46235/1028-7221-16710-IOI
- ID: 16710
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Abstract
Abstract
The goal of researching was to determine the frequency of detection and the structure of causally significant sensitization to the main groups of allergens in children with allergic diseases in Siberia when evaluating the results by enzyme immunoassay and immunofluorescence analysis. We researched 968 children with allergic rhinitis, bronchial asthma, urticaria, allergic dermatitis undergoing treatment at the Consultative and Diagnostic Center for Allergology and Immunology, GDKB No. 2 named after V.P.Bisyarina of Omsk. Blood serum samples were diagnosed In the laboratory of clinical immunology. Also the allergen-specific IgE concentrations were determined for 109 allergens, including food allergens (milk, egg, meat, fish, cereals, fruits, vegetables), household allergens (epidermal pets, dust and house dust mites , molds), inhaled antigens (grass, trees). Researching of 435 children was carried out by enzyme immunoassay, 533 children by indirect immunofluorescence. The research revealed the predominance in children with allergic diseases in Siberia of IgE - mediated sensitization to household allergens, which were detected in 33.7% of the examined. IgE - mediated sensitization to inhalation and food allergens was 1.5 times less common. According to our research the most common causative allergen among households was house dust (90%), dandruff and animal epithelium (86%), house dust mites (13.5%), and molds (1.4%). The most common causative allergen among pollen was tree allergens (47.8%), cereals (23.5%) and weeds (20.1%). The presence of multivalent sensitization with a predominant combination of household and respiratory allergens and a rarer combination of household and food allergens has been experimentally established.
About the authors
Elena L. Shcheglova
Omsk State Medical University, Omsk, Russia
Email: eleon_74@mail.ru
SPIN-code: 2665-3433
Candidate of Medical Sciences, Assistant at the Department of Pathophysiology;
Head of KDL
Russian Federation, OmskTatiana F. Sokolova
Omsk State Medical University, Omsk, Russia
Email: tfsokolova@mail.ru
Doctor of Sciences in Medical Sciences, Associate Professor of the Department of Pharmacolody, Clinical Pharmacolody Omsk State Medical University
Russian Federation, OmskEvgeni Yu. Sokolov
Scientific and Clinical Center «Karpovka», St.Peterburg, Russia
Email: tfsokolova@mail.ru
Candidate of Sciences, Psychotherapist of the Scientific and Clinical Center «Karpovka», St.Peterburg
Russian Federation, St.PeterburgYuri V. Emelyanov
Pirogov Russian National Research Medical University, Institute of Clinical Psychology and Social Work, Moskou, Russia
Author for correspondence.
Email: tfsokolova@mail.ru
Candidate of Sciences, Associate Professor of the Department of Psychotherapy ICPSR
Russian FederationReferences
- Akhapkina I.G., Zheltikova T.M. A comparative analysis of the content of house dust mites and their allergens in residential premises of Moscow // Immunology, 2013, no. 2, pp. 108-111(in Russ.)
- Baysheva M.G., Dyachkova A.A., Prokopieva A.A., Khlopenkova O.V. Сharacteristics of allergic diseases and disability of patients of the prevalence with bronchial asthma in the republic of mordovia for the period 2019–2021//Clinical Hospital, 2023, no 4 (39), pp 5-9. (in Russ.)
- Znamenskaya L.K., Trishina C.V., Gostishheva E.V., Regan O.V. Application experience allergen-specific immunotherapy (ASIT) with the drug antipollin mixed weed herbs-2 in the treatment of seasonal allergic rhinitis caused by weed pollen // Taurida of the Medical and Biological Herald, 2023, Vol. 26, no 1, pp. 22-26. (in Russ.)
- Melnikova KS, Kuwshinowa ED, Reviakina VA. Allergic diseases at an early age. Pediatrics. Consilium Medicum. 2021, no 2, pp. 141–145 (in Russ.) doi: 10.26442/26586630.2021.2.200813
- Namazova-Baranova L. S., Snovskaya M. A., Mityushin I. L., Kozhevnikova O. V., Batyrova A. S. Features of the diagnosis of allergies in children. Bulletin of RAMS, 2017, Vol. 72, no 1, pp. 33–41. (in Russ.)
- Pampura A.N., Varlamov E.E., Konyukova N.G. Sensitization to pet allergens. Ross Western Perina Tol and pediatrician, 2018, Vol. 63, no 2, pp. 22-26. (in Russ.)
- Petrova S.Yu., Khlgatian S.V., Berzhets V.M., Emelyanova O.Yu. Ten-year monitoring of sensitization to house dust mites. Journal of microbiology, epidemiology and immunobiology = Zhurnal mikrobiologii, èpidemiologii i immunobiologii, 2021, 98: online-first. (in Russ.) DOI: https://doi.org/10.36233/0372-9311-49
- Perevoshchikova N.K., Gladkov S.F., Chernykh N.S. Possibilities for the prevention of allergic diseases, taking into account regional characteristics // Mother and child in Kuzbass, 2017, Vol. 69, no. 2, pp. 38-46. (in Russ.)
- Khabibulina L.R., Vlasova N.V., Manzhos M.V., Kavelenova L.M. Blood analysis of allergic components in the comprehensive diagnosis of hay fever and predicting the effectiveness of allergen-specific immunotherapy // Allergology, Immunology, 2019, no. 3, pp. 7-10. (in Russ.)
- Asarnoj A., Hamsten C., Waden K., Lupinek C., Andersson N, Kull I. et al. Sensitization to cat and dog allergen molecules in childhood and prediction of symptoms of cat and dog allergy in adolescence: A BAMSE/MeDALL study. J Allergy Clin Immunol, 2016, Vol. 137, no 3, pp. 813-821. DOI: 10.1016/j. jaci.2015.09.052.
- Konradsen J.R., Fujisawa T, van Hage M., Hedlin G, Hil-ger C., Kleine-Tebbe J. et al. Allergy to furry animals: New insights, diagnostic approaches, and challenges. J Allergy Clin Immunol, 2015, Vol. 135, no 3, pp. 616-625. DOI: 10.1016/j. jaci.2014.08.026.
- Pyrhonen K., Nayha S., Laara E. Dog and cat exposure and respective pet allergy in early childhood. Pediatr Allergy Immunol., 2015, Vol.26, no. 3, pp. 247-255. doi: 10.1111/pai.12369.