LEVELS OF ANTIBODY TITERS TO HERPES SIMPLEX VIRUS (HSV) AND CYTOMEGALOVIRUS INFECTION (CMV) AND CHARACTERISTICS OF THE VAGINAL MICROBIOTA IN WOMEN WITH CHRONIC CERVICITIS AND REPRODUCTIVE DISORDERS

Abstract

The increasing incidence of pelvic inflammatory diseases; latent, asymptomatic course of inflammation, difficulties in isolating the infectious agent, contribute to the search for new diagnostic markers to confirm the etiologic factor. Objective: to assess the levels of antibodies to HSV and CMV infection and to characterize the vaginal microbiome in women with chronic cervicitis and reproductive disorders.

Methods. The prospective study included 72 patients aged 18-45 years. A questionnaire, general clinical, gynecological and instrumental examinations were conducted. Bacteriological, polymerase chain reaction method; serological study were used. Ethical principles were observed, the study was approved by the local biomedical ethics committee. Statistical processing of the obtained data was performed using the StatSoft Statistica 6.1 application package.

Results. In the group as a whole, the frequency of class G antibodies to HSV was 79%, and CMV infection - 92% (p> 0.05). In the structure of the vaginal microbiome, a combination of microaerophiles with mycoplasma representatives was most common in 33%; a combination with HPV was noted in 26%; in 19% it was represented by a combination with candidal infection. In the group of patients with complaints of pathological leucorrhoea (45%), the frequency of Ig G titers over 3200 to HSV type 1.2 was 84% ​​and Ig G titers over 3200 to CMV - 41% (p<0.001). Representatives of the intestinal group were observed in the vaginal microbiome (p<0.001). In asymptomatic chronic cervicitis, Ig G titers over 3200 to CMV infection were observed in 25% and Ig G titers over 3200 to HSV in 68% of patients (p < 0.001). The microbiome is represented by symbiotic microorganisms (p < 0.001) and facultative anaerobic gram-positive microorganisms.

Conclusions. High serological prevalence of specific class G antibodies (Ig G) to HSV and CMV infection indicates the presence of persistent herpes infection. In the presence of pathological leucorrhoea, the microbiome is represented by intestinal microorganisms. Asymptomatic course of chronic cervicitis was accompanied by the presence of candidal infection in symbiosis with ureaplasma and gardnerella, and facultative anaerobic gram-positive microorganisms.