THE EFFECT OF BACTERIAL POLYAMINES ON THE PRODUCTION OF IMMUNOGLOBULINS G IN PATIENTS WITH MAXILLOFACIAL PHLEGMON

AbstractPutrescine and cadaverine are biogenic polyamines that are formed as a result of the breakdown of the amino acids ornithine and lysine, respectively, and, as a rule, with the participation of bacteria. It was revealed that putrescine and cadaverine are able to influence the functional activity of innate immune cells in the focus of the inflammatory process. The ability of these compounds to interact with cells of the immune system, changing their activity, including synthetic activity, causes interest in studying the production of immunoglobulins by plasma cells in the presence of polyamines especially with inflammation. The aim of research was to evaluate the effect of cadaverine and putrescine on the production of immunoglobulin G in the culture of mononuclear leukocytes of patients with maxillofacial phlegmon. Materials and methods. Leukocytes were obtained from 13 patients with maxillofacial phlegmon and 10 healthy donors. After washing the cells, they were introduced into the wells, where B-cell mitogen (PWM) and polyamines were found in concentrations of 5, 25, 50, 75 and 100 mmol/L. An equal volume of nutrient medium was added to the control wells instead of polyamines. At the end of cultivation, a supernatant was collected, where the concentrations of immunoglobulins G (IgG) were determined using the enzyme immunoassay method. Results. In the absence of polyamines, higher IgG production by mononuclears of patients with acute purulent process (27.6±2.2 IU per 1 g of protein) was revealed compared with healthy donors (13.6±1.5 IU per 1 g of protein; p=0.002) with a positive correlation. When culturing patients' lymphocytes in the presence of polyamines, it was found that cadaverine in all concentrations did not significantly change IgG production in mononuclear culture. When using putrescine, it was found that this metabolite in the culture of mononuclears in dose-dependent manner reduces their IgG production. Conclusion. Putrescine and cadaverine have a multidirectional effect on IgG production in the culture of mononuclear leukocytes of patients with facial phlegmon and its due to its role in inflammatory.