COMPARISON OF METHODS CELLULAR IMMUNITY DETECTION TO MUMPS VIRUS



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Abstract

Abstract

Vaccination against the mumps virus has significantly reduced, but not eliminated, the incidence of this infection. Periodically, there are increases in the incidence even among populations with high vaccination coverage. Immunity to the mumps virus is most often assessed by the serum level of specific antibodies, but it is known that T-cell immunity is more important for antiviral protection. The aim of the study was to compare two methods for assessing cellular immunity to mumps virus antigens in intact adults and those who had had this infection. The study included 20 adults who had had mumps (Group 1) and 17 people who had not had or been vaccinated against this infection (Group 2). Antibodies to the mumps virus were determined by ELISA. Cellular immunity was assessed by ELISpot and by the percentage of CD8hiCD107a+ lymphocytes after recognizing mumps virus antigens. None of the subjects in group 2 were found to have IgG antibodies or cellular immunity using the ELISpot method or the percentage of CD8hiCD107a+. In group 1, 2 of 20 subjects were found to have no IgG antibodies to the mumps virus, and 18 subjects had such antibodies at different levels. A high level of cellular immunity to the mumps virus in 10 subjects (9.44±1.27) was detected by ELISpot, and a low level was detected in another 10 subjects (3.44±0.54). Based on CD107a expression on CD8hi lymphocytes, cellular immunity to the mumps virus was not detected in 4 out of 20 individuals, but antibodies to this virus were detected; another 4 individuals had a low level (2.35 (1.9-2.86)%), and 12 individuals had a high level of cellular immunity to the mumps virus (7.72-14.24)%). The cut-off level of 3.76% was calculated, dividing high and low levels of cellular immunity to mumps virus antigens with 100% sensitivity and specificity. Comparison of the ELISpot method and determination of CD107a molecule expression on CD8hi showed a negative correlation r= -0.81, which is associated, firstly, with different populations participating in cellular immunity reactions detected by these two methods, and secondly, ELISpot evaluates IFNγ producers, and CD107a expression evaluates cells that responded to antigen recognition with a cytotoxic attack. Both methods are suitable for studying cellular immunity to mumps virus antigens.

About the authors

Polina E. Zherdeva

G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia

Email: polya-zherdeva@mail.ru
SPIN-code: 1675-7056

Research Associate, Laboratory of Applied Immunochemistry

Russian Federation, 10 Admiral Makarov Street, Moscow, 125212, Russia

Anna P. Toptygina

G.N.Gabrichevsky Research Institute for Epidemiology and Microbiology, Moscow, Russia;
Lomonosov Moscow State University

Email: toptyginaanna@rambler.ru
ORCID iD: 0000-0002-9981-4762
SPIN-code: 8523-5018
Scopus Author ID: 6602424818
ResearcherId: В-6725-2019

DSc (Medicine), Head Research, Head of the Laboratory of Cytokines; Professor, Department of Immunology

Russian Federation, 10 Admiral Makarov Street, Moscow, 125212, Russia

Elena L. Semikina

Federal State Autonomous Institution "National Medical Research Center of Children's Health" of the Ministry of Health of the Russian Federation;
Federal State Autonomous Educational Institution of Higher Education I.M. Sechenov First Moscow State Medical University of the Ministry of Health of the Russian Federation

Author for correspondence.
Email: semikinaelena@yandex.ru
ORCID iD: 0000-0001-8923-4652
SPIN-code: 3647-4967

DSc (Medicine), Head Research, Head of Laboratory Department,   Professor, Department of Pediatrics and Pediatric Rheumatology Pediatric faculty

Russian Federation, 2/1 Lomonosovsky Ave, Moscow, 119991; 8/2, st. Trubetskaya, Moscow, 119991

References

  1. № ссылки Авторы, название публикации и источника, где она опубликована, выходные данные ФИО, название публикации и источника на английском языке Интернет-адрес цитируемой статьи
  2. Агафонов А. П., Пьянков С.А., Рябчикова Е. И., Агафонова О.А., Иванова Л.К., Козловский Л.И., Гинько З.И., Дутов В.Н., Демина О.К., Лосев М.В., Нечаева Е.А., Нетесов С.В., Сергеев А.Н., Дроздов И.Г. Эпидемический паротит. Современные представления о возбудителе, клиника, диагностика, профилактика. /Под ред. А. П. Агафонова. - Новосибирск: ЗАО “Медикобиологический Союз”, 2007. - 82 с Agafonov A.P., Pyankov S.A., Ryabchikova E.I., Agafonova O.A., Ivanova L.K., Kozlovsky L.I., Ginko Z.I., Dutov V.N., Demina O .K., Losev M.V., Nechaeva E.A., Netesov S.V., Sergeev A.N., Drozdov I.G. Mumps. Modern ideas about the pathogen, clinical picture, diagnosis, prevention. /Ed. A. P. Agafonova. - Novosibirsk: JSC “Medicobiological Union”, 2007. - 82 p. (In Russ.) https://mbu.ru/assets/files/parotit.pdf
  3. Смердова М.А., Топтыгина А.П., Андреев Ю.Ю., Сенникова С.В., Зеткин А.Ю., Клыкова Т.Г., Беляков С.И. Гуморальный и клеточный иммунитет к антигенам вирусов кори и краснухи у здоровых людей // Инфекция и иммунитет. 2019. Т. 9, № 3–4. С. 607–611. Smerdova M.A., Toptygina A.P., Andreev Yu.Yu., Sennikova S.V., Zetkin A.Yu., Klykova T.G., Belyakov S.I. Humoral and cellular immunity to measles and rubella virus antigens in healthy subjects // Russian Journal of Infection and Immunity = Infektsiya i immunitet, 2019, Vol. 9, no. 3–4, pp. 607–611. (In Russ.) doi: 10.15789/2220- 7619-2019-3-4-607-611
  4. Топтыгина А.П., Алешкин В.А. Сопоставление первичного и вторичного гуморального иммунного ответа на вакцинацию «Приорикс»// Инфекция и иммунитет, 2013. Т. 3, № 4. С. 359-364 Toptygina A.P., Alioshkin V.A. Comparison of the primary and secondary humoral immune response to vaccinacion by “Priorix”. Russian Journal of Infection and Immunity = Infektsiya i immunitet, 2013, Vol. 3, no.4, pp.359-364 (In Russ.) doi: 10.15789/2220-7619-2013-4-359-364
  5. Топтыгина А.П., Семикина Е.Л., Алешкин В.А. Формирование и поддержание специфического клеточного иммунного ответа на вакцинацию Приорикс. Иммунология. – 2013. – Т.34,№5. – С.257-261 Toptygina A.P., Semikina E.L., Alioshkin V.A. The shaping and the maintenance of T-cell specific immune response to vaccination Priorix. Immunology = Immunologiya, 2013, Vol. 34, no. 5, pp. 257–261. (In Russ.) https://www.elibrary.ru/item.asp?id=20781516
  6. Тураева Н.В., Фролов Р.А., Цвиркун О.В., Герасимова А.Г. Характеристика эпидемиологической ситуации по эпидемическому паротиту в мире на современном этапе. //Эпидемиология и Вакцинопрофилактика. - 2021.- №20(2). – С. 74–82 Turaeva N.V., Frolov R.A., Tsvirkun O.V., Gerasimova A.G. Characteristics of the epidemiological situation of mumps in the world atthe present stage. Epidemiology and Vaccinal Prevention=Epidemiologiya i vaktsinoprofilaktika, 2021, Vol.20, no.2, pp.:74-82. (In Russ.) doi: 10.31631/2073-3046-2021-20-2-74-82
  7. De Laval F., Haus R., Spiegel A., Simon F. Lower long-term immunogenicity of mumps component after MMR vaccine. Pediatr. Infect. Dis J, 2010, Vol. 29(11), pp. 1062-1063. doi: 10.1097/INF.0b013e3181f2d864
  8. de Wit J., Emmelot M.E., Poelen M.C.M., van Binnendijk R.S., van der Lee S., van Baarle D., Han W.G.H., van Els C., Kaaijk P. Mumps infection but not childhood vaccination induces persistent polyfunctional CD8 T-cell memory. J Allergy Clin Immunol., 2018, Vol.141, pp.1908 –1911. doi: 10.1016/j.jaci.2017.11.047
  9. Fields V.S., Safi H., Waters C., Dillaha J., Capelle L., Riklon S., Wheeler J.G., Haselow D.T. Mumps in a highly vaccinated Marshallese community in Arkansas, USA: an outbreak report. Lancet Infect Dis, 2019, Vol. 19(2), pp. 185–192. doi: 10.1016/S1473-3099(18)30607-8
  10. Gouma S., Ten Hulscher H.I., Schurink-van’t Klooster T.M., de Melker H.E., Boland G.J., Kaaijk P., van Els C.A.C.M., Koopmans M.P.G., van Binnendijk R.S. Mumps-specific cross-neutralization by MMR vaccine-induced antibodies predicts protection against mumps virus infection. Vaccine, 2016, Vol. 34, pp.4166 – 4171. doi: 10.1016/j.vaccine.2016.06.063
  11. Han W.G., Emmelot M.E., Jaadar H., Ten Hulscher H.I., van Els C.A., Kaaijk P. Development of an IFN-γ ELISPOT for the analysis of the human T cell response against mumps virus. J Immunol Methods, 2016, Vol.431, pp.52–59. doi: 10.1016/j.jim.2016.02.010
  12. Keshavarz M., Shafiee A., Hossein M., Khosravani P., Yousefi A., Izad M. Immune Response to the Mumps Virus in Iranian Unvaccinated Young Adults. Jpn J Infect Dis., 2017, Vol. 70(2), pp.127-131. doi: 10.7883/yoken.JJID.2016.078
  13. Rubin S., Kennedy R., and Poland G., Emerging Mumps Infection. Pediatr. Infect. Dis. J, 2016, Vol. 35(7), pp. 799–801 doi: 10.1097/INF.0000000000001182
  14. Shepersky L., Marin M., Zhang J., Pham H., Marlow M.A. Mumps in Vaccinated Children and Adolescents: 2007-2019. Pediatrics, 2021, Vol. 148(6), e2021051873. doi: 10.1542/peds.2021-051873
  15. Wang L., Haralambieva I. H., Ovsyannikova I. G., Grill D. E., Warner N. D., Poland G. A., Kennedy R. B. Associations of adaptive immune cell subsets with measles, mumps, and Rubella− Specific immune response outcomes. Heliyon, 2023,Vol. 9, e22998. doi: 10.1016/j.heliyon.2023.e22998

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