REGULATION OF ANTI-CYTOKINE ACTIVITY OF MICROORGANISMS ISOLATED IN POST-OPERATIVE COMPLICATIONS BY SYNTHETIC PEPTIDE ZP2



Cite item

Full Text

Abstract

Abstract

The synthetic analogue of the active center of granulocyte-macrophage colony stimulating factor (GM-CSF), the ZP2 peptide, has a wide range of immunobiological effects, including antibacterial activity. However, its effect (in sub-inhibitory concentrations) on the biological properties of gram-negative bacteria of various species remains poorly understood.  The aim of this study was to compare the effect of the synthetic peptide ZP2– an analog of the active center of GM-CSF, on the anti-cytokine activity of gram-negative bacteria of various species isolated during postoperative complications. Materials and methods.  The study used 42 clinical isolates of gram-negative bacteria of various species (Escherichia coli, Klebsiella pneumoniae, Citrobacter braakii, C. freundii, Stenotrophamonas maltophilia, Pseudomonas aeruginosa, P. putida) isolated from patients with postoperative complications. In the experiment, microorganisms were grown in meat-peptone broth (BCH) with synthetic peptide ZP2 at 37 °C for 24 hours. The anti-cytokine activity (ACA) against IL-4, IL-8, IL-1RA and TNFα was determined by enzyme immunoassay (ELISA). To evaluate ACA, the proportion of cytokine inactivation in the experiment relative to the control was calculated and expressed in pg/ml.   

Results. In vitro experiments investigated the ability of clinical isolates of gram-negative bacteria, isolated during postoperative complications, to inactivate some pro- and anti-inflammatory cytokines (IL-4, IL-8, IL-1RA, TNFα) and demonstrated its dependence on the genus/species of microorganisms.

It was shown that the synthetic peptide ZP2 was characterized by an inhibitory and indifferent effect on the ability of clinical strains of microorganisms to inactivate the studied pro- and anti-inflammatory cytokines. It has been established that ZP2 has a modifying potential that can change both the prevalence and severity of the anti-cytokine activity of clinical isolates of gram-negative bacteria isolated during postoperative complications. 

Conclusions.

  1. Gram-negative bacteria isolated during postoperative complications are capable of secreting compounds that inactivate cytokines IL-4, IL-8, IL-1RA, TNFa.
  2. Synthetic peptide ZP2 in sub-inhibitory concentrations has a modifying effect on the manifestation of anticytokine activity by the studied microorganisms. The modifying effects of synthetic peptide ZP2 revealed in vitro experiments expand the range of its potential clinical application.

About the authors

Olga Pashinina

Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia

Email: olga25mikro@mail.ru

Candidate of Biological Sciences, Senior Researcher of the Laboratory of Microbial Persistence and Symbiosis ICIS Ural Branch of the Russian Academy of Sciences – a separate structural unit of the FSBI OFRC Ural Branch of the Russian Academy of Sciences

Russian Federation

Tatyana M. Pashkova

Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia

Email: pashkova070782@mail.ru

PhD, MD (Biology), Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms, Institute of Cellular and Intracellular Symbiosis, Ural Branch, Russian Academy of Sciences

Russian Federation, Orenburg

Viktor A. Gritsenko

Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia

Author for correspondence.
Email: vag59@mail.ru

PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms

Russian Federation, Orenburg

References

  1. Бухарин О.В., Перунова Н.Б., Чайникова И.Н., Иванова Е.В., Смолягин А.И. Антицитокиновая активность микроорганизмов // Журнал микробиологии, эпидемиологии и иммунобиологии. – 2011. – № 4. – С. 56-61. Buharin O.V., Perunova N.B., Chajnikova I.N., Ivanova E.V., Smolyagin A.I. Anti-cytokine activity of microorganisms. Journal of Microbiology, Epidemiology and Immunobiology. 2011, no. 4, pp. 56-61.
  2. //medj.rucml.ru/journal/45562d4d4943524f42494f4c435249452d41525449434c452d3133353235/
  3. Гриценко В.А., Тяпаева Я.В., Добрынина М.А., Зурочка А.В. Сравнительный анализ бактерицидных свойств синтетического пептида активного центра ГМ-КСФ – ZP2 в отношении грамотрицательных бактерий разной таксономической принадлежности // Российский иммунологический журнал. – 2021. – Т.24, №2. – С. 221-228. Gritsenko V.A., Tyapaeva Ya.V., Dobrynina M.A., Zurochka A.V. Comparative analysis of bactericidal properties of the synthetic peptide of the active center of GM-CSF – ZP2 in relation to gram-negative bacteria of different taxonomic affiliation. Russian Journal of Immunology. 2021, Vol.24, no. 2, pp. 221-228.
  4. doi: 10.46235/1028-7221-1016-CAO
  5. Зурочка А.В., Добрынина М.А., Зурочка В.А., Гриценко В.А. Чувствительность музейных и клинических штаммов энтеробактерий к синтетическому пептиду активного центра ГМ-КСФ – ZP2 // Российский иммунологический журнал. – 2020. – Т.23, №4. – С. 403-410. Zurochka A.V., Dobrynina M.A., Zurochka V.A., Gricenko V.A. Sensitivity of museum and clinical strains of enterobacteria to the synthetic peptide of the active center of GM-CSF – ZP2. Russian Journal of Immunology, 2020, Vol. 23, no. 4, pp. 403-410.
  6. doi: 10.46235/1028-7221-503-SOA
  7. Зурочка А.В., Зурочка В.А., Добрынина М.А., Гриценко В.А. Иммунобиологические свойства гранулоцетарно-макрофагального колониестимулирующего фактора и синтетических пептидов его активного центра // Медицинская иммунология. – 2021. – Т.23, №5. – С. 1031-1054.
  8. Zurochka A.V., Zurochka V.A., Dobrynina M.A., Gritsenko V.A. Immunobiological properties of granulocyte-macrophage colony-stimulating factor and synthetic peptides of its active center. Medical Immunology, 2021, Vol.23, no. 5, pp. 1031-1054.
  9. doi: 10.15789/1563-0625-IPO-2216
  10. Зурочка А.В., Гриценко В.А., Зурочка В.А., Добрынина М.А., Черешнев В.А. Гранулоцитарно-макрофагальный колониестимулирующий фактор (ГМ-КСФ) и его синтетические аналоги: иммунобиологические эффекты и клиническое применение. – Екатеринбург: УрО РАН, 2021. – 288 с. Zurochka A.V., Gricenko V.A., Zurochka V.A., Dobrynina M.A., Chereshnev V.A. Granulocyte-macrophage colony-stimulating factor (GM-CSF) and its synthetic analogues: immunobiological effects and clinical application. Ekaterinburg: Ural Branch of the Russian Academy of Sciences, 2021, 288 p.
  11. ISBN 978-5-7691-2549-2
  12. Кузьмин М.Д., Пашкова Т.М., Карташова О.Л., Пашинина О.А., Попова Л.П. Биологические свойства микроорганизмов, выделенных из мочи при мочекаменной болезни // Урология. – 2018. – №4. – С. 14-18. Kuzmin M.D., Pashkova T.M., Kartashova O.L., Pashinina O.A., Popova L.P. Biological properties of microorganisms isolated from urine in urolithiasis. Urology. 2018. no. 4, pp. 14-18.
  13. doi: 10.18565/urology.2018.4.14-18
  14. Пашинина О.А., Пашкова Т.М., Карташова О.Л., Морозова Н.В. Антицитокиновая активность грибов рода Candida и их способность к продукции цитокиноподобных веществ // Российский иммунологический журнал. – 2023. – Т.26, №1. – С. 57-62. Pashinina O.A., Pashkova T.M., Kartashova O.L., Morozova N.V. Anti-cytokine activity of Candida fungi and their ability to produce cytokine-like substances. Russian Journal of Immunology, 2023, Vol. 26, no. 1, pp. 57-62.
  15. doi: 10.46235/1028-7221-1150-AAO
  16. Пашинина О.А., Карташова О.Л., Пашкова Т.М., Гриценко В.А., Зурочка А.В. Влияние синтетического аналога активного центра ГМ-КСФ – пептида ZP2 на антицитокиновую активность грибов рода Candida и их способность к продукции цитокиноподобных веществ // Вестник Уральской медицинской академической науки. – 2022. – Т.19, №3. – С. 263-272. Pashinina O.A., Kartashova O.L., Pashkova T.M., Gricenko V.A., Zurochka A.V. The effect of a synthetic analogue of the active center of GM-CSF – peptide ZP2 on the anti-cytokine activity of Candida fungi and their ability to produce cytokine-like substances. Bulletin of the Ural Medical Academic Science, 2022, Vol. 19, no. 03, pp. 263-272.
  17. doi: 10.22138/2500-0918-2022-19-3-263-272
  18. Пашинина О.А., Карташова О.Л., Пашкова Т.М., Гриценко В.А. Влияние синтетического аналога активного центра ГМ-КСФ – пептида ZP2 на антицитокиновую активность бактерий рода Enterococcus и их способность к продукции цитокиноподобных веществ // Российский иммунологический журнал. – 2022. – Т.25, №4. – С. 477-484.
  19. Pashinina O.A., Kartashova O.L., Pashkova T.M., Gricenko V.A. The effect of a synthetic analogue of the active center of GM-CSF – peptide ZP2 on the anti-cytokine activity of bacteria of the genus Enterococcus and their ability to produce cytokine-like substances. Russian Journal of Immunology, 2022, Vol. 25, no. 4, pp. 477-484.
  20. doi: 10.46235/1028-7221-1162-IOZ
  21. Карташова О.Л., Пашкова Т.М., Пашинина О.А., Морозова Н.В. Антицитокиновая активность и способность к продукции цитокиноподобных веществ стафилококков, выделенных из секрета предстательной железы у мужчин с симптомами урогенитальной инфекции // Российский иммунологический журнал. – 2021. – Т. 24, №2. – С. 257-260. Kartashova O.L., Pashkova T.M., Pashinina O.A., Morozova N.V. Anti-cytokine activity and ability to produce cytokine-like substances of staphylococci isolated from prostatic secretion in men with symptoms of urogenital infection. Russian Journal of Immunology, 2021, Vol. 24, no. 2, pp. 257-260.
  22. doi: 10.46235/1028-7221-995-AAA

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) Pashinina O., Pashkova T.M., Gritsenko V.A.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies