RED CLOVER (TRIFOLIUM PRATENSE) ISOFLAVONES REDUCE OXIDATIVE STRESS IN THE SPLEEN AND LIVER OF RATS WITH EXPERIMENTAL TYPE 2 DIABETES



Cite item

Full Text

Abstract

Abstract

Type 2 diabetes (T2D) is a chronic disease with a large number of severe complications. The quantity of patients is large and continues to grow worldwide, so development of new means of prevention and treatment are extremely relevant. Oxidative stress caused by hyperglycemia is one of the main factors of organ damage and dysfunction in T2D. Given the close involvement of the immune system in the pathogenesis of diabetes and its complications, the possibility of influencing the activity of the antioxidant defense system in the organs of immunopoiesis, in particular, spleen, is of interest. The liver, which integrates all types of metabolism, along with the organs of the immune system, both contributes to the pathogenesis of T2D and is its target. Red clover (Trifolium pratense) is of interest as a cheap and accessible source of antioxidants. The aim of the work was to evaluate the effect of red clover isoflavones on the antioxidant defense system in blood, spleen and liver of rats with experimental diabetes. Materials and methods. For a month, three times a week, sexually mature male rats with a streptozotocin-nicotinamide model of T2D were orally administered aqueous extracts of isoflavones, obtained by “green technology” methods using deep natural eutectic solvents, at doses of 300 and 600 mg/kg. The glucose content, the relative content of glycated hemoglobin and the number of leukocytes of different fractions were measured in blood. The activity of enzymes of the antioxidant defense system catalase and superoxide dismutase, the content of the lipid peroxidation product malondialdehyde and the endogenous antioxidant reduced glutathione were biochemically determined in blood, liver and spleen. Results. The administration of red clover flower isoflavone extracts (Trifolium pratense) reduced hyperglycemia and restored the number of monocytes and granulocytes in the blood, reduced in rats with diabetes; normalized the activity of catalase in the liver and the activity of superoxide dismutase in the spleen, the level of malondialdehyde in the spleen and liver and the content of reduced glutathione in the spleen, changed in rats with diabetes, to healthy control values, regardless of the dose. Conclusions. Red clover flower (Trifolium pratense) isoflavone extracts attenuate hyperglycemia and reduce oxidative stress in the organs of rats with experimental T2D. Isoflavone extracts have an immunopharmacological effect, manifested in the effect on the parameters of the lipid peroxidation-antioxidant defense system in the spleen.

About the authors

D. A. Tukanov

Ural Federal University named after the first President of Russia B.N. Yeltsin, Ekaterinburg, Russia;
Institute of Immunology and Physiology UrB RAS, Ekaterinburg, Russia

Email: danil_tukanov@mail.ru
ORCID iD: 0009-0002-1197-5885

graduate student of the Institute of Immunology and Physiology of the Ural Branch of the Russian Academy of Sciences; Junior Researcher, Laboratory of Functional Design of Nanocluster Polyoxymetallates, Institute of Natural Sciences and Mathematics, Ural Federal University named after the first President of Russia B.N. Yeltsin

Russian Federation, 106, Pervomayskaya str., Yekaterinburg, 620049, Institute of Immunology and Physiology UB RAS

K. V. Sokolova

Ural Federal University named after the first President of Russia B.N. Yeltsin, Ekaterinburg, Russia;
Institute of Immunology and Physiology UrB RAS, Ekaterinburg, Russia

Author for correspondence.
Email: xenia.socolova@gmail.com
ORCID iD: 0000-0002-7024-4110
SPIN-code: 6057-5630
ResearcherId: AAO-6863-2021

PhD (Biology), Research Associate, Laboratory of Advanced Materials, Green Methods and Biotechnologies, Chemical-Technological Institute, Senior Research Associate, Laboratory of Morphology and Biochemistry

Russian Federation, Yekaterinburg; Yekaterinburg

References

  1. Volkhina I.V., Butolin E.G. Oxidative stress and changes in liver sialoglycoconjugate metabolic parameters in rats with alloxanic diabetes mellitus. Diabetes mellitus. 2022, Vol. 25, no. 3, pp. 249-255. https://doi.org/10.14341/DM12763
  2. Emelianov V.V., Gette I.F., Danilova I.G., Sidorova L.P., Zeitler T.A., Mukhlynina E.A. Effect of 1,3,4-thiadiazine class compounds on the severity of oxidative stress in experimental type 2 diabetes mellitus. Chemical and pharmaceutical journal. 2023. Vol. 57, No. 3. P. 20-24. doi.org/10.30906/0023-1134-2023-57-3-20-24
  3. Sokolova K.V., Gette I.F., Tukanov D.A., Danilova I.G. Peculiarities of oxidative stress in the liver and spleen of rats with experimental type 2 diabetes mellitus. [Online] Vestn. Ural. Med. Akad. Nauki. = Journal of Ural Medical Academic Science. 2024, Vol. 21, no. 5, pp. 565-573. doi: 10.22138/2500-09182024-21-5-565-573
  4. Aboushanab S.A., Shevyrin V.A., Melekhin V.V., Andreeva E.I., Makeev O.G., Kovaleva E.G. Cytotoxic Activity and Phytochemical Screening of Eco-Friendly Extracted Flavonoids from Pueraria montana var. lobata (Willd.) Sanjappa & Pradeep and Trifolium pratense L. Flowers Using HPLC-DAD-MS/HRMS // AppliedChem. 2023; 3(1):119-140. https://doi.org/10.3390/appliedchem3010009
  5. Akbaribazm M., Khazaei M.R., Khazaei M. Phytochemicals and antioxidant activity of alcoholic/hydroalcoholic extract of Trifolium pretense // Chinese Herbal Medicines, Volume 12, Issue 3, 2020, Pages 326-335, https://doi.org/10.1016/j.chmed.2020.02.002
  6. Caturano A, D'Angelo M, Mormone A, Russo V, Mollica MP, Salvatore T, Galiero R, Rinaldi L, Vetrano E, Marfella R, Monda M, Giordano A, Sasso FC. Oxidative Stress in Type 2 Diabetes: Impacts from Pathogenesis to Lifestyle Modifications. Curr Issues Mol Biol. 2023 Aug 12;45(8):6651-6666. doi: 10.3390/cimb45080420
  7. Dias, H.F., Fu, J.F., Luck, T.G. et al. The spleen assumes a major role in blood glucose regulation in type 1 diabetes patients treated with BCG. Sci Rep 14, 17611 (2024). https://doi.org/10.1038/s41598-024-67905-x
  8. Hanchang W, Wongmanee N, Yoopum S, Rojanaverawong W. Protective role of hesperidin against diabetes induced spleen damage: Mechanism associated with oxidative stress and inflammation. J Food Biochem. 2022 Dec;46(12):e14444. doi: 10.1111/jfbc.14444
  9. Janssen LMM, Hiligsmann M, Elissen AMJ, Joore MA, Schaper NC, Bosma JHA, Stehouwer CDA, Sep SJS, Koster A, Schram MT, Evers SMAA. Burden of disease of type 2 diabetes mellitus: cost of illness and quality of life estimated using the Maastricht Study. Diabet Med. 2020 Oct;37(10):1759-1765. doi: 10.1111/dme.14285
  10. Jasvinder Singh Bhatti, Abhishek Sehrawat, Jayapriya Mishra, Inderpal Singh Sidhu, Umashanker Navik, Naina Khullar, Shashank Kumar, Gurjit Kaur Bhatti, P. Hemachandra Reddy Oxidative stress in the pathophysiology of type 2 diabetes and related complications: Current therapeutics strategies and future perspectives // Free Radical Biology and Medicine. – 2022. – Vol. 184, pp. 114-134, https://doi.org/10.1016/j.freeradbiomed.2022.03.019
  11. Masiello P., Broca C., Gross R., [et al.] Experimental NIDDM: development of a new model in adult rats administered streptozotocin and nicotinamide. Diabetes. – 1998. – Vol. 47 (2). – P. 224-9
  12. Mora-Ortiz, M., Nuñez Ramos, P., Oregioni, A. et al. NMR metabolomics identifies over 60 biomarkers associated with Type II Diabetes impairment in db/db mice. Metabolomics 15, 89 (2019). https://doi.org/10.1007/s11306-019-1548-8

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) Tukanov D.A., Sokolova K.V.

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies