HUMORAL IMMUNE RESPONSE TO C. KEFIRRESIDENTII AND S. EPIDERMIDIS AS PATHOBIOTIC COMPONENTS OF TUBERCULOSIS FOCI



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Abstract

Abstract

The role of lung microbiota in tuberculosis has attracted increasing attention, particularly in the context of its interaction with the immune system and its contribution to systemic chronic inflammation. Microorganisms constituting the local microbial environment of tuberculosis lesions may not only generate specific immune signals but also participate in the immunopathogenesis of infection through cross-interactions with the host immune system.

In a previously conducted large-scale microbiological screening of facultative anaerobic microbiota in tuberculosis lesions, we isolated two bacterial strains – Corynebacterium kefirresidentii and Staphylococcus epidermidis – from the caseous material of tuberculomas. Based on their microbiological and biochemical characteristics, as well as identified resistance to several first-line antituberculosis drugs, these microorganisms are considered elements of the pathobiota that may contribute to the maintenance of local inflammation in tuberculosis. Cell lysates of these microorganisms were used as sources of potential antigens to assess immune responses.

In the present study, a laboratory approach was developed and tested to comparatively evaluate the humoral immune response to components of nonspecific microbiota associated with tuberculosis lesions. The analysis was carried out using a semi-quantitative ELISA with blood serum samples obtained from patients with pulmonary tuberculosis (n = 90) and healthy donors (n = 90). Seroprevalence and levels of specific IgG against C. kefirresidentii were significantly higher in tuberculosis patients compared to the control group (p < 0.05), suggesting a possible involvement of this microorganism in the immunopathogenesis of the disease. In contrast, no statistically significant differences were observed between groups for S. epidermidis, which is likely related to its high prevalence in the general population as a common skin commensal. Although the role of immune responses to S. epidermidis in tuberculosis has not been previously considered, some studies indicate the involvement of certain strains in the pathogenesis of common dermatological disorders. A positive correlation between antibody levels to both microorganisms, observed only in tuberculosis patients (p = 0.001), may indicate a pathological nature of immune interactions in pulmonary tuberculosis. These findings highlight the importance of evaluating humoral responses to microbiota components in the context of tuberculosis and expand our understanding of immune mechanisms accompanying this infectious process.

About the authors

Elizaveta A. Orlova

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Author for correspondence.
Email: elizaveta.a.orlova@gmail.com
ORCID iD: 0000-0003-2169-0242
SPIN-code: 5258-4087
Scopus Author ID: 57223019914
ResearcherId: ABC-3428-2020

Junior Research Officer at the Laboratory of Epidemic and Social Infections

Russian Federation, 664003, Russian Federation, Irkutsk, Timiryazeva st., 16

Oleg B. Ogarkov

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Email: obogarkov@mail.ru
ORCID iD: 0000-0002-3168-1983
SPIN-code: 1956-2888
Scopus Author ID: 6603264302
ResearcherId: G-6966-2011

Doctor of Medical Sciences, Director of the Institute of Epidemiology and Microbiology of the FSBSI SС FHHRP

Russian Federation, 664003, Russian Federation, Irkutsk, Timiryazeva st., 16

Lyubov I. Kolesnikova

Scientific Center for Family Health and Human Reproduction Problems, Irkutsk, Russian Federation

Email: kolesnikova20121@mail.ru
ORCID iD: 0000-0003-3354-2992
SPIN-code: 1584-0281
Scopus Author ID: 7005230276

Doctor of Medical Sciences, Academician of the Russian Academy of Sciences, Professor, Scientific Director of the FSBSI SС FHHRP

Russian Federation, 664003, Russian Federation, Irkutsk, Timiryazeva st., 16

References

  1. Гульнева М.Ю. Особенности гуморального иммунного ответа на антигены оппортунистических микроорганизмов у больных системной красной волчанкой // Актуальные проблемы медицины, 2021. Т. 44, № 3. С. 278-285 Gulneva M.Yu. Features of the humoral immune response to antigens of opportunistic microorganisms in patients with systemic lupus erythematosus. Aktual'nye problemy mediciny = Challenges in Modern Medicine, 2021, Vol. 44, no. 3, pp. 278-285. (In Russ.) 10.52575/2687-0940-2021-44-3-278-285
  2. Огарков О.Б., Суздальницкий А.Е., Кондратов И.Г., Букин Ю.С., Орлова Е.А., Синьков В.В., Жданова С.Н., Белькова Н.Л., Рычкова Л.В., Колесникова Л.И. Выделение и полногеномное секвенирование липофильной анаэробной бактерии, представителя видового комплекса Corynebacterium tuberculostearicum, из туберкулёзного очага // Acta Biomedica Scientifica, 2023. Т. 8, № 4. С. 12-19. Ogarkov O.B., Suzdalnitsky A.E., Kondratov I.G., Bukin Yu.S., Orlova E.A., Sinkov V.V., Zhdanova S.N., Belkova N.L., Rychkova L.V., Kolesnikova L.I. Isolation and whole genome sequencing of a lipophilic anaerobic bacterium, a representative of the species complex Corynebacterium tuberculostearicum, from a tuberculosis focus. Acta Biomedica Scientifica, 2023, Vol. 8, no. 4, pp. 12-19. (In Russ.) 10.29413/ABS.2023-8.4.2
  3. Орлова Е.А., Огарков О.Б., Колесникова Л.И. Особенности микробиоты легких при туберкулезной инфекции // Бюллетень сибирской медицины, 2024. Т. 23, № 1. С. 166-175 Orlova E.A., Ogarkov O.B., Kolesnikova L.I. Features of the lung microbiota in tuberculosis infection. Byulleten' sibirskoj mediciny = Bulletin of Siberian Medicine, 2024, Vol. 23, no. 1, pp. 166-175. (In Russ.) 10.20538/1682-0363-2024-1-166-175
  4. Синьков В.В., Орлова Е.А., Огарков О.Б., Суздальницкий А.Е., Кондратов И.Г., Белькова Н.Л., Рычкова Л.В. Геном Staphylococcus epidermidis из казеозного некроза туберкулемы // Генетика, 2024. Т. 60, № 10. С. 129-134 Sinkov V.V., Orlova E.A., Ogarkov O.B., Suzdalnitsky A.E., Kondratov I.G., Belkova N.L., Rychkova L.V. The genome of Staphylococcus epidermidis Isolated from Caseous Tuberculoma. Genetika = Russian Journal of Genetic, 2024, Vol. 60, no. 10. pp. 129-134. (In Russ.) doi: 10.31857/S0016675824100139
  5. Henriksson G., Bredberg J., Wullt M., Lyrenäs E., Hindorf U., Ohlsson B., Grip O. Humoral response to Clostridium difficile in inflammatory bowel disease, including correlation with immunomodulatory treatment. JGH Open, 2018, Vol. 3, no. 2, pp. 154-158. – 10.1002/jgh3.12122
  6. Lardeux F., Torrico G., Aliaga C. Calculation of the ELISA’s cut-off based on the change-point analysis method for detection of Trypanosoma cruzi infection in Bolivian dogs in the absence of controls. Mem. Inst. Oswaldo. Cruz, 2016, Vol. 111, no. 8, pp. 501-504. – 10.1590/0074-02760160119
  7. Matucci A., Maggi E., Vultaggio A. Cellular and humoral immune responses during tuberculosis infection: useful knowledge in the era of biological agents. J. Rheumatol Suppl., 2014, Vol. 91, pp. 17-23. – 10.3899/jrheum.140098
  8. Ramírez-Labrada A.G., Isla D., Artal A., Arias M., Rezusta A., Pardo J., Gálvez E.M. The influence of lung microbiota on lung carcinogenesis, immunity, and immunotherapy. Trends Cancer, 2020, Vol. 6, no. 2, pp. 86-97. – 10.1016/j.trecan.2019.12.007.
  9. Rijnink W.F., Ottenhoff T.H.M., Joosten S.A. B-cells and antibodies as contributors to effector immune responses in tuberculosis. Frontiers in Immunology, 2021, Vol. 12, 640168. – 10.3389/fimmu.2021.640168
  10. Severn M.M., Horswill A.R. Staphylococcus epidermidis and its dual lifestyle in skin health and infection. Nat. Rev. Microbiol., 2023, Vol. 21, no. 2, pp. 97-111. – 10.1038/s41579-022-00780-3
  11. Stoll M.L., Kumar R., Morrow C.D., Lefkowitz E.J., Cui X., Genin A., Cron R.Q., Elson C.O. Altered microbiota associated with abnormal humoral immune responses to commensal organisms in enthesitis-related arthritis. Arthritis Res. Ther., 2014, Vol. 16, no. 1, 486. – 10.1186/s13075-014-0486-0

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