DEFENSINES IN PATIENTS WITH ISCHEMIC STROKE

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Abstract

The question of the importance of inflammatory and immune mechanisms of brain damage in cerebrovascular disease remains relevant.

The aim of our study was to assess the level of inflammation and the anti-bacterial potential of neutrophilic granulocytes (NG) in patients with ischemic stroke (IS). Surveyed 17 people with IS aged 68.5 (67; 79) years and 15 volunteers aged 65.0 (62.0; 66.5) years. The neurological status of the patients was assessed using the NIHSS and Rankin scales, Rivermid Mobility Index. Blood IL-6, CRP, cortisol, fibrinogen, soluble defensins (s Def) levels at 1, 3 and 10 days after IS was determined. α-Defensins in NG (def+ NG) were evaluated usingm Abs HNР 1–3 (NCL-Defensin). To assess the phagocytic activity of NG, the phagocytic index (PI) and phagocytic number (PN) were determined. Statistical processing of the research results was carried out using STATISTIKA 6.0.

Main results. Signs of systemic inflammation were observed in patients with IS blood levels of NG, IL-6, CRPand cortisol. The relationship of the somatic status of patients, the size of the lesion of the brain tissue, the severity of IS, the degree of disability, the outcome of IS with the content of NG and def+ NG was revealed. Phagocytosis inhibition in the dead was associated with a degree of disability. A positive association between def+ NG and NG (r = 0.659, p <0.001), IL-6 (r = 0.659, p ><0.001), cortisol (r = 0.573, p ><0.001), fi brinogen (r = 0.369, p >< 0.05). The data obtained indicate the development of systemic inflammation and an imbalance of the functional activity of NG in patients with IS, which are associated with the severity of cerebral stroke and create the risk of developing infectious complications. 

About the authors

L. P. Pivovarova

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

Email: fake@neicon.ru

doctor of medical sciences, head of the laboratory diagnostics department,

Saint-Petersburg

Russian Federation

I. V. Osipova

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

Author for correspondence.
Email: ivosipova@yandex.ru

PhD, Senior Researcher, Laboratory Diagnostics Department,

Saint-Petersburg

Russian Federation

O. B. Ariskina

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

Email: fake@neicon.ru

PhD in Biology, Research Assistant of the Laboratory Diagnostics Department,

Saint-Petersburg

Russian Federation

E. A. Gogoleva

Saint-Petersburg I. I. Dzhanelidze Research Institute of Emergency Medicine

Email: fake@neicon.ru

Head of the Neurology Department for Patients with Acute Сerebral Сirculation Disorders,

Saint-Petersburg

Russian Federation

References

  1. Chamorro A., Urra X., Planas A. M. Infection after acute ischemic stroke: a manifestation of brain-induced immunodepression. Stroke.2007;38:1097–1103.
  2. Ruhnau J., Schulze K., Gaida B., Langner S., Kessler C., Bröker B., Dressel A., Vogelgesang A. Stroke alters respiratory burst in neutrophils and monocytes. Stroke. 2014; Mar;45(3):794–800.
  3. Ganz T. Defensins: antimicrobial peptides of innate immunity.Nat Rev Immunol. 2003; Sep; 3 (9):710–20.

Copyright (c) 2019 Pivovarova L.P., Osipova I.V., Ariskina O.B., Gogoleva E.A.

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