EFFECT OF MACROPHAGE CONDITION MEDIA ON PHENOTYPE AND PROLIFERATION OF HUMAN NEUROBLASTOMA CELL LINE SH-SY5Y

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Authors: Rashchupkin I.M.¹, Tikhonova M.A.¹, Tyrinova T.V.¹, Sakhno L.V.¹, Shevela E.Y.¹
Affiliations:
1. Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»
Issue: Vol 22, No 2-2 (2019)
Pages: 903-905
Section: ORIGINAL ARTICLES
URL: https://rusimmun.ru/jour/article/view/390
DOI: https://doi.org/10.31857/S102872210006548-7
ID: 390

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Abstract
About the authors
References
Supplementary files
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Abstract

The study investigated the effect of conditioned media of M1 and M2 macrophages on the phenotype and proliferation of the human neuroblastoma cell line SH-SY5Y. It has been established that conditioned media of M2 macrophages, generated in the presence of GM-CSF under defi ciency of growth factors, have a pronounced stimulating effect on the SH-SY5Y cell proliferation. The expression of the low-affinity nerve growth factor receptor, CD271, correlates with cell morphology: in cultures with a predominance of N-type cells, the number of CD271+ cells is signifi cantly higher compared to cultures dominated by S-type cells.

Keywords

M1 and M2 macrophages, macrophage-conditioned medium, neuroblastoma cell line SH-SY5Y

About the authors

I. M. Rashchupkin

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Author for correspondence.
Email: iwwwanbets@mail.ru

second year residentof the Laboratory of Cellular Immunotherapy,

Novosibirsk

Russian Federation

M. A. Tikhonova

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

Russian Federation

T. V. Tyrinova

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD (Medicine), Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

Russian Federation

L. V. Sakhno

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD (Biology), Senior Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

Russian Federation

E. Ya. Shevela

Federal Budget Institution of Science «Research Institute of Fundamental and Clinical Immunology»

Email: fake@neicon.ru

PhD, MD (Medicine), Leading Research Associate, Laboratory of Cellular Immunotherapy,

Novosibirsk

Russian Federation

References

Hu X., Leak R.K., Shi J., Suenaga J., Gao Y., Zheng P., Chen J. Microglial and macrophage polarization – new prospects for brain repair. Nat Rev Neurol. 2015,11(1), 56–64.
Sakhno L.V., Shevela E.Ya., Tikhonova M. A., Ostanin A. A, Chernykh E. R. Scandinavian journal of Immunology. 2015. 83, 151–159.
Ferlemann F. C., Menon V., Condurat A. L., Rößler J., Pruszak J. Surface marker profiling of SH-SY5Y cells enables small molecule screens identifying BMP4 as a modulator of neuroblastoma differentiation. Sci Rep. 2017, 7(1), 1–14.

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Copyright (c) 2019 Rashchupkin I.M., Tikhonova M.A., Tyrinova T.V., Sakhno L.V., Shevela E.Y.

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This work is licensed under a Creative Commons Attribution 4.0 International License.

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