Email this article MONOCLONAL ANTIBODY TO HUMAN PD-1 END DETERMINATION OF ITS SPECIFIC ACTIVITY BY FLOW CYTOMETRY
- Authors: Isaeva E.N.1, Sineva S.A.1, Pigareva N.V.1, Simbirtsev A.S.1
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Affiliations:
- FGUP ”State Research Institute of Highly Pure Biopreparations” Federal Agency for Medicine and Biology
- Issue: Vol 22, No 3 (2019)
- Pages: 1173-1176
- Section: ORIGINAL ARTICLES
- Submitted: 26.08.2020
- Accepted: 26.08.2020
- Published: 15.10.2019
- URL: https://rusimmun.ru/jour/article/view/498
- DOI: https://doi.org/10.31857/S102872210007249-8
- ID: 498
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Abstract
The cell receptor PD-1 is a member of the CD28. This coinhibitory receptor is a member of subfamily “immunological checkpoints”. For this reason, testing the activities of such preparations is important for clinical medicine. In this study, a human monoclonal anti PD-1 antibody was developed and tested for functional activity by flow cytometry immunophenotyping analysis. This method was demonstrated to be eff ective for protein identifi cation and for fast screening of monoclonal antibody functional activity.
About the authors
E. N. Isaeva
FGUP ”State Research Institute of Highly Pure Biopreparations” Federal Agency for Medicine and Biology
Author for correspondence.
Email: e.n.isaeva@hpb.spb.ru
candidat of biology, biologist laboratory of protein biochemistry,
St. Petersburg
Russian FederationS. A. Sineva
FGUP ”State Research Institute of Highly Pure Biopreparations” Federal Agency for Medicine and Biology
Email: fake@neicon.ru
lead biologist, laboratory of protein biochemistry,
St. Petersburg
Russian FederationN. V. Pigareva
FGUP ”State Research Institute of Highly Pure Biopreparations” Federal Agency for Medicine and Biology
Email: fake@neicon.ru
candidat of biology, lead biologist, laboratory of protein biochemistry,
St. Petersburg
Russian FederationA. S. Simbirtsev
FGUP ”State Research Institute of Highly Pure Biopreparations” Federal Agency for Medicine and Biology
Email: fake@neicon.ru
MD, corresponding member of RAS, Professor, of scientific adviser,
St. Petersburg
Russian FederationReferences
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