THE LEVEL OF INTERLEUKIN17 AND INTERFERON γ IN CELL’S CULTURES SUPERNATANTS UNDER STIMULATION BY ANTIBODIES TO CD3 AND CD28 IN CHILDREN WITH ARTHRITIS OF DIFFERENT ETIOLOGY

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Abstract

Children of 2–17 years old with juvenile idiopathic arthritis (n = 100), unspecified reactive arthropathy (n = 15) and 31 healthy children (controls) were examined. The spontaneous, anti-CD3-stimulated and anti-CD3/CD28-stimulated concentrations of IL-17 and IFN-γ in the cells cultures supernatants were determined by ELISA. The spontaneous IFN-γ level in children with arthritis of various etiologies was higher than in control, while the IFN-γ concentrations in both variants of stimulation in the patients were lower. The spontaneous IL-17 production in the patients and in healthy children was the same, while stimulated synthesis of IL-17 in the groups with arthritis was higher than in the control group.

About the authors

I. A. Pashnina

Regional Children’s Clinical Hospital

Author for correspondence.
Email: irina_pashnina@list.ru

PhD, Head of clinical diagnostics laboratory,

 

Russian Federation

I. M. Krivolapova

Regional Children’s Clinical Hospital

Email: fake@neicon.ru

biologist of clinical diagnostics laboratory,

 

Russian Federation

References

  1. Клиническая лабораторная диагностика: национальное руководство: в 2 т. / под ред. В. В. Долгова, В. В. Меньшикова. М.: ГЭОТАР-Медиа, 2012. 808 с.
  2. Симбирцев А. С. Цитокины в патогенезе инфекционных и неинфекционных заболеваний человека. Медицинский академический журнал 2013, 13 (3), 18–41.
  3. Журавлева Ю. А., Соломатина Л. В., Гусев Е. Ю. Особенности цитокинемии при различных аутоиммунных заболеваниях. Российский иммунологический журнал 2017, 11 (20), (2), 315–316.
  4. Гатторно М. Аутовоспалительные заболевания у детей. Вопросы современной педиатрии 2014, 13 (2), 55–64.
  5. Алексеева Е. И., Литвицкий П. Ф. Ювенильный ревматоидный артрит: этиология, патогенез, клиника, алгоритмы диагностики и лечения, под ред. А. А. Баранова. М.: ВЕДИ. 2007. 368 с.
  6. Rypdal V., Arnstad E. D., Aalto K., Berntson L., Ekelund M., Fasth A., Glerup M., Herlin T., Nielsen S., Peltoniemi S., Zak M., Rygg M., Rypdal M., Nordal E. Predicting unfavorable long-term outcome in juvenile idiopathic arthritis: results from the Nordic cohort study. Arthritis Research & Therapy, 2018, 20:91.
  7. Gorska A., Kowal-Bielecka O., Urban M., Pietrewicz E. Interleukin-6 and tumor necrosis factor alpha levels in the serum and synovial fluid in relation to bone mineral density and turnover in children with juvenile idiopathic arthritis. Eur. J. Immunology 2008, 33 (4), 216–219.
  8. Pietrewicz E., Urban M., Górska A. Cytokine levels in serum of patients with juvenile idiopathic arthritis depending on subtype and disease activity. Pol. Merkur. Lekarski 2004, 17 (99), 232–234.
  9. Yılmaz M., Kendirli S.G., Altıntas D., Bing G., Antmen B. Cytokine levels in serum of patients with juvenile rheumatoid arthritis. Clinical Rheumatology 2001, 20 (1), 30–35.
  10. Kohler K., Ganser A., Andre T., Roth G., Grosse-Hovest L., Jung G., Brock R. Stimulus dependence of the action of small-molecule inhibitors in the CD3/CD28 signalling network. Chem. Med. Chem. 2008, 3 (9), 1404–1411.
  11. Trimble L. A., Shankar P., Patterson M., Daily J. P., Lieberman J. Human Immunodeficiency Virus-Specific Circulating CD8 T Lymphocytes Have DownModulated CD3 and CD28, Key Signaling Molecules for T-Cell Activation. Journal Of Virology 2000, 74, 16, 7320–7330.
  12. Agarwal S., Misra R., Aggarwal A. Interleukin 17 levels are increased in juvenile idiopathic arthritis synovial fluid and induce synovial fibroblasts to produce proinflammatory cytokines and matrix metalloproteinases. J Rheumatol. 2008, 35 (3), 515–519.
  13. Beringer A., Noack M., Miossec P. IL-17 in Chronic Inflammation: From Discovery to Targeting. Trends Mol. Med. 2016, 22 (3), 230–241.
  14. Chen K., Kolls J. K. Interleukin-17A (IL17A). Gene 2017, 30 (614), 8–14.
  15. Szymańska-Kałuża J., Cebula-Obrzut B., Smolewski P., Stanczyk J., Smolewska E. Imbalance of Th17 and Tregulatory cells in peripheral blood and synovial fluid in treatment naïve children with juvenile idiopathic arthritis. Central European Journal of Immunology 2014, 39 (1), 71–76.
  16. Vij atov-Djuric G., Doronjski A., Mitic I. Brkic S., Barisic N. Interleukin-17A Levels Increase in Serum of Children With Juvenile Idiopathic Arthritis. Arch Rheumatol. 2017, 32 (10), 1–10.
  17. Sebbag M., Parry S. L., Brennan F. M., Feldmann M. Cytokine stimulation of T lymphocytes regulates their capacity to induce monocyte production of tumor necrosis factor-alpha, but not interleukin-10: possible relevance to pathophysiology of rheumatoid arthritis. Eur. J. Immunol. 1997, 27 (3), 624–632.
  18. Moller B., Paulukat J., Nold M., Behrens M., KukocZivojnov N., Kaltwasser J. P., Pfeilschifter J., Mühl H. Interferon-γ induces expression of interleukin-18 binding protein in fibroblast-like synoviocytes. Rheumatology 2003, 42 (3), 442–445.
  19. Пашнина И. А. Спонтанная и стимулированная фитогемагглютинином экспрессия маркеров активации CD25, CD38 и CD69 Т-лимфоцитами при заболеваниях различной этиологии у детей. Российский иммунологический журнал 2017, 11 (20), (3), 465–467.
  20. Пашнина И. А. Экспрессия CD69 на Т-лимфоцитах у детей с аутоиммунными заболеваниями при стимуляции через Т-клеточный рецептор или через Т-клеточный рецептор и ко-рецепторную молекулу CD28. Российский иммунологический журнал 2014, 8 (17), (3), 579–581.
  21. Криволапова И. М., Пашнина И. А., Черешнев В. А. Уровень провоспалительных цитокинов в супернатантах культур цельной крови у детей с ювенильным идиопатическим артритом. Вестник Уральской медицинской академической науки 2018, 15 (3), 421–431.

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