FEATURES OF CYTOKINE PRODUCTION EX VIVO IN CHILDREN UNDER STRONTIUM EXPOSURE

The features of spontaneous and induced synthesis of cytokines ex vivo in children under strontium exposure were analyzed. The levels of spontaneous, mitogen-induced (phytohemagglutinin 4 μg/ml, concavalin A 4 μg/ml, lipopolysaccharide 2 μg/ml) and strontium (0.01 mg/ml) cytokine production in supernatants of whole peripheral blood of children living in the endemic area of stable strontium exposure were determined by enzyme immunoassay. When compared to the spontaneous production of cytokines by peripheral blood cells of children in the observation group, we revealed a significant decrease in IL-12 levels by 3.1 times, TNF-α by 5.6 times regarding the comparison group and the absence of differences in the spontaneous synthesis of IL-6, IL-10, IL-17, GM-CSF. Under the conditions of inducing effect of strontium, a decrease in production of TNF-α, IL-6, IL-12, IL-17, GM-CSF was found to be by 5.0, 1.7, 2.4, 1.6, 5.0 times, respectively, at the overall stimulating effect of the metal on the indicators of the observation group, IL-6 by 133.1 times, IL-12 by 1.4 times, GM-CSF by 2.2 times, TNF-α by 1.8 times. The data obtained indicate a modulating effect of strontium on cytokine production in whole blood cultures with a predominantly activating effect on mediators of the pro-infl ammatory action of IL-6, IL-12, GM-CSF, TNF-α.

immune regulation, cytokines, strontium