BLOOD LEUKOCYTE PHAGOCYTIC AND CYTOKINE-PRODUCING ACTIVITY OF ANTI-PLAGUE VACCINATED BALB/C LINE MICE AGAINST THE BACKGROUND OF IMMUNOMODULATION BY POLYOXIDONIUM

Polyoxidonium increases the immunogenicity and protection of the live plague vaccine, but the cellular mechanisms underlying its immunomodulating effect are not well understood. In present study, the blood granulocyte phagocytic activity (PA) of anti-plague vaccinated and not vaccinated BALB / c mice in relation to Yersinia pestis, Escherichia coli and Staphylococcus aureus was investigated using flow cytometry. The results of the phagocytic reaction were taken into account in the microvolumes of whole blood on days 3, 7 and 21 of immunogenesis. Spontaneous and CoA-induced cytokine production (IFN-γ and IL-10) was evaluated in the blood by an ELISA method. In one of the groups of animals, anti-plague vaccination was carried out against the background of immunomodulation with a polyoxidonium. It was established that in intact mice the blood granulocyte PA in relation to Y. pestis cells is twice as low as in experiments with E. coli and S. aureus cells. Anti-plague vaccination specifically activated in vitro the blood granulocyte absorptive capacity only in relation to plague microbes. Against the background of the polyoxidonium immunomodulating effect, PA increased in experiments with Y. pestis cells already on the 3rd day of immunogenesis, which is consistent with the known ability of this immunomodulator to stimulate earlier antibody genesis during anti-plague vaccination. In addition, an increase in phagocytic index values correlated with an increase in spontaneous and induced cytokine production in blood samples of animals vaccinated against the plague. The data obtained reflect the dependence of blood leukocyte PA from the pathogen type, specific antibodies and cytokines. They indicate that the polyoxidonium ability to increase the live plague vaccine protectivity may be associated with the activation of leukocyte PA to plague microbes.