INFLUENCES OF HEXAPEPTIDE ARGINYL-ALPHA-ASPARTIL-LIZIL-VALYL-TYROSIL-ARGININ ON THE TRANSFORMED PHENOTYPE OF NEUTROPHILIC GRANULOTSITES OF HEALTHY PERSONS IN THE EXPERIMENTAL SYSTEM INVITRO

The experimental model of the in vitro transformed phenotype of neutrophilic granulocytes (NG) equipped with receptors CD64, CD32, CD16, CD11b, was created under the influence of fMLPs. The features of the transformed phenotype of NG were described. The influence of the Hexapeptide arginyl-alpha-aspartyl-lysyl-poured-tyrosyl-arginine (HP) on untransformed and transformed phenotype of NG was investigated in vitro. It was shown that fMLP increases the number of NG, expressing the receptor CD64, increases the density of expression of the receptor CD11b, and moderately reduces the amount of CD32⁺ NG. HP does not influence on the number of NG, bearing receptors CD64, CD32, CD16, CD11b, and the level of their expression. At the same time with simultaneous incubation with fMLP NG and HP, are shown to mitigate the effects of fMLP: reduced the number of CD64⁺ NG, reduced the density of CD11b expression, decreases in the number of NG bearing a receptor CD32. Overall, the results demonstrate the classic immunomodulatory effect HP: on the one hand, the absence of any change in the studied receptors of NG under his influence and leveling the transformational changes of phenotypic characteristics of NG, which arose under the influence of fMLP.

нейтрофилы, трансформированный фенотип, fMLP, neutrophils, the transformed phenotype, fMLP