CD56 EXPRESSION BY PERIPHERAL BLOOD CYTOTOXIC T CELLS IN MULTIPLE SCLEROSIS

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Abstract

Multiple Sclerosis (MS) is a chronic inflammatory autoimmune disease of the central nervous system. Recent reports have shown CD3+CD8+ and their different subsets take part in neuroinflammation. During the current study frequency, phenotype as well as CD56 and CD57 expression of peripheral blood CD3+CD8+ cells derived from healthy volunteers (n = 52), MS subjects during relapse (n = 31) or remission (n = 20) were characterized using ten-color flow cytometry. CD3+CD8+ were divided into naïve, central (CM) and effector memory (EM), as well as effector cells (TEMRA) based on the expression of CD45RA, CD62L, CD27 and CD28. No differences were observed during the comparison of N, CM and TEMRA CD3+CD8+ subsets relative and absolute number between the groups, but the number of CM cells was decreased in MS groups The relative and absolute number of “naïve” CD3+CD8+ cells were inversely related (r = –0,512, р = 0,003 and r = –0,430, р = 0,014, respectively) to the degree of disability of examined patients. The percentages of CD57+ cells were increased only within naïve and CM subsets in both MS groups compared with healthy volunteers. While the relative numbers of CD56+ cells were significantly evaluated in all investigated cytotoxic T cell subsets in MS group with the exception of TEMRA cells from patients during remission in comparison with control group. Our data demonstrate that CD56 level on CD3+CD8+ subsets may represent a biomarker of neuroinflammation in MS patients.

About the authors

I. V. Kudryavtsev

Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”

Author for correspondence.
Email: igorek1981@yandex.ru

PhD, senior researcher, department of immunology

197376 St. Petersburg, acad. Pavlov str., 12 Russian Federation

A. G. Ilves

N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences

Email: fake@neicon.ru

PhD, senior researcher, laboratory of neuroimmunology

St. Petersburg Russian Federation

O. M. Novoselova

N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences

Email: fake@neicon.ru

junior researcher, laboratory of neurorehabilitation

St. Petersburg Russian Federation

K. S. Rubanik

Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”

Email: fake@neicon.ru

junior researcher, laboratory of neurorehabilitation

St. Petersburg Russian Federation

M. K. Serebriakova

Federal State Budgetary Scientific Institution “Institute of Experimental Medicine”

Email: fake@neicon.ru

Research Associate, department of immunology

St. Petersburg Russian Federation

L. N. Prakhova

N.P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences

Email: fake@neicon.ru

MD, Head of the laboratory of neurorehabilitation

St. Petersburg

Russian Federation

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Copyright (c) 2018 Kudryavtsev I.V., Ilves A.G., Novoselova O.M., Rubanik K.S., Serebriakova M.K., Prakhova L.N.

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