Modulation of functional activity of mast cells by cholinergic agents

Cover Page


Cite item

Full Text

Abstract

Being immunocompetent cells, mast cells at the same time have a number of properties characteristic of cells of the nervous system, which is why they are often viewed as key intermediaries between the integrating systems of the body. There is an increasing interest of the scientifi c community in the anti-infl ammatory activity of the parasympathetic nervous system. Despite this, the question of the mechanisms of cholinergic regulation of mast cells has not been studied suffi ciently. As a model of mast cells, we used the HMC-1 human mast line cells. The results of our study showed that carbachol (Cch), a functional analog of acetylcholine, simultaneously exhibits properties of the histamine liberator and the suppressor of IgG-induced activation of mast cells. This dual eff ect of the neurotransmitter may indicate the presence of a cholinergic tone supporting the excitation of mast cells at a certain level. The modulating eff ect of Cch on the functional activity of HMC-1 cells is realized through nicotinic acetylcholine receptors (n-AChR). The data obtained contribute to deepening the concept of the mechanisms of negative regulation of mast cells.

About the authors

N. . Kutukova

Institute of Experimental Medicine

Author for correspondence.
Email: noemail@neicon.ru
Russian Federation

P. . Nazarov

1Institute of Experimental Medicine; The I.P. Pavlov First St. Petersburg State Medical University

Email: noemail@neicon.ru
Russian Federation

References

  1. Быков В. Л. Цитология и общая гистология. Сотис, СПб, 2007, 519 с. [Bykov V. L. Cytology and General Histology. St. Petersburg: Sotis, 2007, 519 p.].
  2. Gilfi llan A. M., Austin S. J., Metcalfe D. D. Mast cell biology: introduction and overview. Adv. Exp. Med. Biol. 2011, 716, 2–12.
  3. Bauer O., Razin E. Mast cell-nerve interactions. News Physiol. Sci. 2000, 15, 213–18.
  4. Kle? H. P., Bienenstock J. Signifi cance of conversation between mast cells and nerves. Allergy Asthma Clin. Immunol. 2005, 1 (2), 65–80.
  5. Forsythe P., Bienenstock J. The mast cell-nerve functional unit: a key component of physiologic and pathophysiologic response. Chem. Immunol. Allergy 2012, 98, 196–221.
  6. Moon T. C., St Laurent C. D., Morris K. E., Marcet C., Yoshimura T., Sekar Y., Befus A. D. Advances in mast cell biology: new understanding of heterogeneity and function. Mucosal Immunol. 2010, 3 (2), 111–28.
  7. Guzman-Mejia F., Lopez-Rubalcava C., Gonzalez-Espinosa C. Stimulation of nAchR?7 receptor inhibits TNF synthesis and secretion in response to LPS treatment of mast cells by targeting ERK1/2 and TACE activation. J. Neuroimmune Pharmacol. 2018, 13 (1), 39–52.
  8. Tore F., Tuncel N. Mast cells: target and source of neuropeptides. Curr. Pharm. Des. 2009, 15 (29), 3433–3445.
  9. Borovikova L. V., Ivanova S., Zhang M., Yang H., Botchkina G. I., Watkins L. R., Wang H., Abumrad N., Eaton J. W., Tracey K. J. Vagus nerve stimulation attenuates the systemic infl ammatory response to endotoxin. Nature 2000, 405 (6785), 458–462.
  10. Tracey K. J. The infl ammatory refl ex. Nature 2002, 420 (6917), 853–859.
  11. Wang H., Liao H., Ochani M., Justiniani M., Lin X., Yang L., Al-Abed Y., Wang H., Metz C., Miller E. J., Tracey K. J., Ulloa L. Cholinergic agonists inhibit HMGB1 release and improve survival in experimental sepsis. Nat. Med. 2004, 10 (11), 1216–1221.
  12. Huston J. M., Ochani M., Rosas-Ballina M., Liao H., Ochani K., Pavlov V. A., Gallowitsch-Puerta M., Ashok M., Czura C. J., Foxwell B., Tracey K. J., Ulloa L. Splenectomy inactivates the cholinergic antiinfl ammatory pathway during lethal endotoxemia and polymicrobial sepsis. J. Exp. Med. 2006, 203 (7), 1623–1628.
  13. Shore P. A., Burkhalter A., Jr. Cohn V. H. A method for the fl uorometric assay of histamine in tissues. J. Pharmacol. Exp. Ther. 1959, 127, 182–186.
  14. Sudheer P. S., Hall J. E., Donev R., Read G., Rowbottom A., Williams P. E. Nicotinic acetylcholine receptors on basophils and mast cells. Anaesthesia 2006, 61(12), 1170–1174.
  15. Radosa J., Dyck W., Goerdt S., Kurzen H. The cholinergic system in guttate psoriasis with special reference to mast cells. Exp. Dermatol. 2011, 20 (8), 677–679.
  16. Пронина А. П. Влияние факторов острой фазы воспаления на активацию базофилов и тучных клеток in vitro. Автореф. дисс. канд. биол. наук. СПб 2011, 19 c. [Pronina A. P. Infl uence of acute infl ammation phase factors on the activation of basophils and mast cells in vitro. Abstract. Dissertation for the degree of Cand. Biol. Sci. St. Petersburg, 2011, 19 c.]
  17. Nazarov P. G., Pronina A. P. The infl uence of cholinergic agents on histamine release from HMC-1 human mast cell line stimulated with IgG, C-reactive protein and compound 48/80. Life Sci 2012, 91 (21–22), 1053–1057.
  18. Пронина А. П., Назаров П. Г. Активация базофилов крови человека лигандами Fc-gamma-рецепторов: холинергический тюнинг. Цитокины и воспаление 2008, 7 (4), 40–46. [Pronina A. P., Nazarov P. G. Activation of human blood basophiles by the Fc? receptor ligands: a cholinergic tuning. Cytokines and Infl ammation 2008, 7 (4), 42–48].
  19. Пронина А. П., Назаров П. Г. Влияние пентраксинов и агрегированного IgG на выброс гистамина из базофилов человека при блокаде АХ-рецепторов. Росс. аллергол. журн 2008, 1 (1), 237–238. [Pronina A. P., Nazarov P. G. Eff ect of pentraxines and aggregated IgG on the release of histamine from human basophils in the blockade of acetylcholine receptors. Russian Allergol. J. 2008, 1(1), 237–238.]
  20. Nazarov P. G., Pronina A. P., Trulioff A. S. C-reactive protein: Fc receptor-mediated eff ects on human peripheral blood basophils. In: Basophil Granulocytes. Ed. P. K. Vellis. NY: Nova Science Publishers, 2009, 95–121
  21. Nazarov P. G., Pronina A. P., Trulioff A. S. C-reactive protein: Fc-gamma receptor-mediated eff ects on human peripheral blood basophils in vitro. In: C-Reactive Protein: New Re-search. Ed. S. Nagasawa. NY: Nova Science Publishers, 2009, 147–169.
  22. Reinheimer T., Baumgartner D., Hohle K. D., Racke K., Wessler I. Acetylcholine via muscarinic receptors inhibits histamine release from human isolated bronchi. Am. J. Respir. Crit. Care Med. 1997, 156 (2 Pt 1), 389–395.
  23. Reinheimer T., Mohlig T., Zimmermann S., Hohle K. D., Wessler I. Muscarinic control of histamine release from airways. Inhibitory M1-receptors in human bronchi but absence in rat trachea. Am. J. Respir. Crit. Care Med. 2000, 162(2 Pt 1), 534–538.

Supplementary files

Supplementary Files
Action
1. JATS XML
Download

Copyright (c) 2018 Kutukova N..., Nazarov P...

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).
Регистрационный номер и дата принятия решения о регистрации СМИ: серия ПИ № 77 - 11525 от 04.01.2002.


This website uses cookies

You consent to our cookies if you continue to use our website.

About Cookies