CHANGE OF LEVELS DEFENZINOV IN STOMATIC LIQUID AT PATIENTS AT THE ALLERGIC AND NONSPECIFIC HYPERSENSITIVITY TO STOMATOLOGIC MATERIALS

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Abstract

The purpose of the real research was studying of influence of dental alloys on levels α‑1‑ and β‑1‑ defensin in oral liquid at patients with complaints to intolerance of dentoprosthetic materials and with the revealed sensitization to salts of metals. The studied group 20 patients with intolerance of dentoprosthetic materials and positive have made results of statement of patch test and reaction of allergen induction damage of leukocytes. To patients carried out determination of level of defensin from leukocytes of oral liquid after the transbukkal of provocative tests from 0,001% solutions salts of metals: NiCl2, CrCl3, CoCl2. It is established what patients with intolerance of dentoprosthetic materials of an allergic etiology have (n = 20), proved by application tests with salts of metals, levels α‑1‑ and β‑1‑defensin in oral liquid change in 1 month after removal of causal orthopedic designs. The α‑1‑defensin level in oral liquid before removal of causal orthopedic designs has made 1,56 [1,19; 1,95] ng/ml, and 1 month later after removal – 0,61 [0,44; 0,68] ng/ml (PWilcoxon ≤0,05). Level of a β‑1‑ defensin before removal of artificial limbs has made 2,9 [2,3; 3,4] ng/ml, and after removal of artificial limbs has increased to 3,8 [3,1; 4,9] ng/ml. Patients of control group have levels α‑1‑ and β‑1‑defenzin in oral liquid authentically didn’t change. After carrying out the transbukkal of provocative tests with salts of metals at patients about intolerance of dentoprosthetic materials of an allergic etiology (n = 20) reliable (PWilcoxon = 0,043) increase in level of α‑1‑defensin in oral liquid was observed. However, the gain or decrease in levels of β‑1‑defensin under influence the transbukkal of provocative tests wasn’t observed. The obtained data specify also hyperreactivity of neutrophils in at patients with intolerance of dentoprosthetic materials as granules of neutrophils are a source α‑1‑defensine, and the lowered level of an ephitelium β‑1‑defensin characterizes oppression of mukozal immunity of a mucous membrane of an oral cavity.

About the authors

I. Yu. Karpuk

Vitebsk State Medical University

Author for correspondence.
Email: ikarpuk@mail.ru

PhD, Assistant professor of the Department of Clinical Immunology and Allergy

210029 Vitebsk, ul. Pravdi 66, 112

Belarus

References

  1. Kucukkolbasi H., Kucukkolbasi S., Ayyildiz H. F., Dursun R., Kara H. Evaluation of hbetaD-1 and hbetaD-2 levels in saliva of patients with oral mucosal diseases. West Indian Med. J. 2013 Mar, 62(3), 230–238.
  2. Fu Gorr S.-U. Antimicrobial peptides of the oral cavity. Periodontology 2009, 51, 152–180.
  3. Jana N.K., Gray L. R., Shugars D. C. Human immunodeficiency virus type 1 stimulates the expression and production of secretory leukocyte protease inhibitor (SLPI) in oral epithelial cells: A role of SLPI in innate mucosal immunity. J. Virol. 2005, 79, 6432–6440.
  4. Hollox E. J. Copy number variation of beta-defensins and relevance to disease. Cytogenet. Genome Res. 2008, vol. 123, № 1–4, 148–155.
  5. Rehaume L. M., Hancock R. E. Neutrophil-derived defensins as modulators of innate immune function. Crit. Rev. Immunol. 2008, vol. 28, № 3, 185–200.
  6. Dietrich D.E., Xiao X., Dawson D. V. Human alpha- and beta-defensins bind to immobilized adhesins from Porphyromonas gingivalis. Infect Immun. 2008, 76, 5714–5720.
  7. Baines K. J., Wright T. K., Simpson J. L., McDonald V. M., Wood L. G. Airway β-Defensin-1 Protein Is Elevated in COPD and Severe Asthma. Allergy Asthma Clin Immunol. 2016; 12: 52.
  8. Ozturk A., Famili P., Vieira A. R. The antimicrobial peptide DEFB1 is associated with caries. J. Dent. Res. 2010, 89, 631–636.
  9. Nishi Y., Isomoto H., Mukae H., Ishimoto H., Wen C. Y. Concentrations of α- and β-defensins in gastric juice of patients with various gastroduodenal diseases. World J. Gastroenterol. 2005, Jan 7, 11(1), 99–103.
  10. Sawaki K., Mizukawa N., Yamaai T., Fukunaga J., Sugahara T. Immunohistochemical study on expression of alpha-defensin and beta-defensin-2 in human buccal epithelia with candidiasis. Oral Dis. 2002, 8, 37–41.
  11. Ji S., Hyun J., Park E., Lee B. L., Kim K. K. Susceptibility of various oral bacteria to antimicrobial peptides and to phagocytosis by neutrophils. J. Periodontal Res. 2007, 42, 410–419.
  12. Premratanachai P., Joly S., Johnson G. K., McCray P.B., Jr, Jia H.P. Expression and regulation of novel human beta-defensins in gingival keratinocytes. Oral Microbiol. Immunol. 2004, 19, 111–117.
  13. Lu Q., Jin L., Darveau R. P., Samaranayake L. P. Expression of human beta-defensins-1 and -2 peptides in unresolved chronic periodontitis. J. Periodontal. Res. 2004, 39, 221–227.
  14. Shelburne C.E., Coulter W. A., Olguin D., Lantz M. S., Lopatin D. E. Induction of beta-defensin resistance in the oral anaerobe Porphyromonas gingivalis. Antimicrob Agents Chemother. 2005, 49, 183–187.
  15. Колчанова Н.Э., Окулич В. К., Денисенко А. Г. Уровень бета-1-дефензина, бапна-амидазной и эластазной активности ротовой жидкости у пациентов с хроническим периодонтитом Вестник ВГМУ 2016, 15, 2, 102–109.
  16. Новиков Д.К., Сергеев Ю. В., Новиков П. Д. Лекарственная аллергия. М.: Нац. акад. микологии, 2001, 313.

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