Dependence of neutrophiles respiratory burst from the state of their metabolic processes in patients with chronic endometritis and adnexitis

The purpose of this work was to study the dependence of neutrophils respiratory burst from the activity of NAD(P)-dependent dehydrogenases in patients with chronic endometritis and adnexitis. The study involved 89 women of reproductive age with chronic endometritis and adnexitis. As a control were examined 98 healthy women of a similar age range. The respiratory burst condition of blood neutrophils was examined using a chemiluminescent assay. Investigation of the NAD(P)-dependent dehydrogenases activity in neutrophils was carried out using the bioluminescent method. A high intensity of the respiratory explosion of neutrophils was detected in chronic endometritis that was determined by an increase in the synthesis of primary and secondary reactive oxygen species (ROS). In patients with chronic adnexitis a respiratory burst of neutrophils occurs in the imbalance of synthesis of primary and secondary ROS: with a reduced activity of NADPH-oxidase there was a high level of secondary ROS synthesis. Metabolism of neutrophils in women with infectious and infl ammatory diseases of the pelvic organs is characterized by a high level of lipid metabolism and NAD-dependent substrate fl ow between the reactions of the tricarboxylic acid cycle and the amino acid exchange. A feature of neutrophil metabolism in chronic endometritis is the high activity of malate dehydrogenase. Characteristic features of neutrophil granulocyte metabolism in patients with chronic adnexitis are a decrease in the intensity of anaerobic glycolysis and NADP-dependent substrate fl ow between the reactions of the tricarboxylic acid cycle and the amino acid exchange. The relationship between respiratory explosion rates and neutrophil metabolism in chronic endometritis refl ects the inhibitory eff ect of the anaerobic reaction of lactate dehydrogenase on the induced synthesis of primary ROS. The respiratory explosion of neutrophils in women with chronic adnexitis depends on the activity of NADP-dependent dehydrogenases, but with the pronounced eff ect of enzymes in the energy profi le. A characteristic feature of chronic adnexitis also is a stimulating eff ect on the synthesis of primary ROS activity of glutathione reductase. Determination of the metabolic regulation mechanisms of respiratory burst in neutrophils allows us to characterize the regulatory aspects of immunopathogenesis of infectious and infl ammatory diseases and can be the basis for the development of new methods of immunometabolic therapy.