NEUTROPHIL CD64 AND PROCALCITONIN IN THE DIAGNOSIS OF SEPSIS OF DIFFERENT ETIOLOGIES

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Abstract

The article presents the results of investigations of procalcitonin concentrations and the relative amount and density of expression of CD64 on neutrophils in patients with sepsis caused by gram-negative, gram-positive bacteria and Candida. The most pronounced increase in the relative amount of CD64+ neutrophils and expression density of this marker was observed in patients with gram-negative and fungal sepsis. When the relative amount of CD64+ neutrophils more than 83% probability of gram- negative or candidal sepsis is defined with a sensitivity of 87%, a specificity of 81%. Determined a direct correlation between procalcitonin level and nCD64. The level of procalcitonin in the serum of patients with sepsis is also different depending on causative agents: the maximum concentration determined in sepsis caused by gram-negative bacteria, whereas gram-positive and Candida sepsis procalcitonin increase was less pronounced. nCD64 is a highly sensitive and specific marker for bacterial and fungal infections and can be used to assess the effectiveness of therapy. Determination of the expression level nCD64 to diagnose sepsis caused by gram-negative bacteria, and sepsis caused by fungi of the genus Candida.

About the authors

A. A. Kalashnikova

The Federal State Budgetary Institute «The Nikiforov Russian Centre of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disaster

Author for correspondence.
Email: petkova_nas@mail.ru

Candidate of Biological Sciences (Doctor of Philosophy, Ph.D.) Senior Research Officer Laboratory of Clinical Immunology

197345 Saint-Petersburg, ul. Optovikov, 54

Russian Federation

T. M. Voroshilova

The Federal State Budgetary Institute «The Nikiforov Russian Centre of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disaster

Email: fake@neicon.ru

Head of Laboratory of Bacteriological Studies

Saint-Petersburg Russian Federation

M. Yu. Frolova

The Federal State Budgetary Institute «The Nikiforov Russian Centre of Emergency and Radiation Medicine» The Ministry of Russian Federation for Civil Defense, Emergencies and Elimination of Consequences of Natural Disaster

Email: fake@neicon.ru

Candidate of Biological Sciences (Doctor of Philosophy, Ph.D.) Head, Senior Research Officer Laboratory of Clinical Chemistry

Saint-Petersburg Russian Federation

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Copyright (c) 2017 Kalashnikova A.A., Voroshilova T.M., Frolova M.Y.

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