RHEUMATOID REGULATORY FACTOR IN THE BLOOD OF RATS IMMUNIZED WITH AUTOLOGOUS LYMPHOCYTES, ACTIVATED IN VITRO BY HETEROLOGOUS LYMPHOCYTES



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Abstract

Abstract

The search for approaches to solve the problems of acute and chronic transplant rejection, overcoming tissue incompatibility, is one of the important issues of clinical transplantology and regenerative medicine. Induction of tolerance to natural and artificial transplant antigens has been a desired goal for more than a century. It has recently been shown that anti-lymphocytic antibodies to activated lymphocytes, called regulatory rheumatoid factor (regRF), are involved in the control of autoreactive lymphocytes in normal conditions, maintaining natural tolerance. It was noted that a relatively rapid increase in the level of regRF in the blood (3-5 day) after immunization is observed during autotransplantation. Its early rise is also associated with the insensitivity of animals to experimentally induced autoimmune diseases.

The aim of our study was to find out whether it is possible to induce regRF production in rats by introducing autologous lymphocytes activated in vitro by a heterologous antigen (mouse lymphocytes).

To achieve this, one group of rats was immunized intravenously with a mixed culture of autologous and heterologous (mouse) lymphocytes (MLC), after 5 days of co-incubation. The other group was immunized only mouse lymphocytes. RegRF in the blood was determined by 3, 5, 7, 14, 21, 28 and 35 days after immunization. It was hypothesized that the intravenous injection of autologous rat lymphocytes activated in MLC and mouse lymphocytes to rats would cause an early regRF response to autologous lymphocytes and a later one to heterologous mouse lymphocytes. 

According to the average data, the titer of regRF in response to mouse lymphocyte immunization of rats practically did not change, however, only on day 7 there was a significant increase in the standard deviation, which indicates an expressed individual response of rats to the introduction of heterologous mouse lymphocytes. Immunization of animals to MLC caused a two-phase increase in regRF in the blood, on days 5 and 21.

An increase in regRF on day 5 after MLC immunization indicates a reaction to autologous lymphocytes activated by a heterologous antigen. The obtained results open up the prospect for further research aimed at developing methods for inducing acquired tolerance.

About the authors

Anastasia V. Kryazhevskikh

Udmurt Federal Research Center UB RAS

Email: krav526@gmail.com
ORCID iD: 0000-0001-5341-9719
SPIN-code: 7100-7142

Research engineer, Laboratory of Biocompatible Materials

Russian Federation, 34 T. Baramzina St, Izhevsk, 426067; 1 Universitetskaya St, Izhevsk, 426034;

Nadezhda N. Abisheva

Udmurt Federal Research Center UB RAS;
Udmurt State University

Author for correspondence.
Email: naidik84@mail.ru
ORCID iD: 0000-0001-5663-4369
SPIN-code: 8909-9872
Scopus Author ID: 55535455300
ResearcherId: U-4444-2019

PhD (Biology), Senior Research Associate, Laboratory of Biocompatible Materials; Senior Research Associate, Laboratory of Molecular and Cell Immunology

Russian Federation, 34 T. Baramzinoy St, Izhevsk, 426067; 1 Universitetskaya St, Izhevsk, 426034;

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