Email this article COMPARATIVE ANALYSIS OF IMMUNORESISTANCE OF CLINICAL ISOLATES OF ENTEROCOCCI AND STAPHYLOCOCCI IN CHRONIC BACTERIAL PROSTATITIS
- Authors: Pashinina O.A.1, Pashkova T.M.1, Kartashova O.L.1, Popova L.P.1, Kuzmin M.D.1, Gritsenko V.A.1
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Affiliations:
- Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
- Section: Forum Sochi 2025
- Submitted: 30.04.2025
- Accepted: 22.06.2025
- URL: https://rusimmun.ru/jour/article/view/17260
- DOI: https://doi.org/10.46235/1028-7221-17260-CAO
- ID: 17260
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Abstract
Abstract
The aim of the study was to conduct a comparative analysis of immunoresistance factors in enterococci and staphylococci strains isolated in chronic bacterial prostatitis (CBP). In vitro studies were conducted on 24 clinical strains of gram-positive cocci of different species: Enterococcus faecalis (n=6), Staphylococcus aureus (n=6), coagulase-negative staphylococci (CoNS): S. haemolyticus (n=6), S. epidermidis (n=6), isolated from prostatic secretion in men with CBP. Antilysozyme activity (ALA) of bacteria and their ability to form biofilms were determined photometrically; sIgA protease activity of bacteria and their anticytokine activity (ACA) in relation to pro- and anti-inflammatory cytokines (IL-6, IL-8, IL-17A, TNFα and IL-4) were determined by the enzyme immunoassay. The obtained data were processed by the methods of variation statistics and the Spearman rank correlation method.
During the comparative analysis of immunoresistance factors in enterococci and staphylococci isolated in CBP, no significant differences in the expression levels of ALA, BPO, and ACA in relation to IL-8 and TNFα were found between the isolates of bacteria of the studied species, but their interspecies variability in the expression of sIgA protease activity and ACA in relation to IL-4, IL-6, and IL-17A was revealed. The results of the correlation analysis indicated the presence of a close positive relationship between the expression of ACA IL-8 and ACA IL-6 in CoNS and S. aureus isolates, as well as between sIgA protease activity and ACA IL-6 in CoNS and E. faecalis strains. Inverse correlation dependencies between ACA IL-8 and ACA IL-17A, ACA IL-8 and BPO; as well as BPO and ACA TNFα were detected in E. faecalis.
The conducted studies allowed us to characterize a number of immunoresistance factors associated with inactivation/suppression of antimicrobial defense mechanisms in strains of E. faecalis, S. aureus and CoNS of different species isolated from patients with CBP, which can contribute to the survival and long-term persistence of pathogens in the prostate gland. Further studies in this area can provide new practically useful information, including information related to the prediction of persistence of specific strains.
About the authors
Olga A. Pashinina
Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
Email: olga25mikro@mail.ru
ORCID iD: 0000-0001-9944-3095
SPIN-code: 9638-7336
Scopus Author ID: 57196410629
ResearcherId: J-3331-2018
PhD (Biology), Senior Research Associate Laboratory of Microbial Persistence and Symbiosis
Russian Federation, 460000, Orenburg, st. Pioneer, 11
Tatiana M. Pashkova
Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
Email: pashkova070782@mail.ru
ORCID iD: 0000-0001-8075-8249
SPIN-code: 5146-6190
Scopus Author ID: 57189696210
ResearcherId: J-2057-2018
PhD, MD (Biology), Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, 460000, Orenburg, st. Pioneer, 11Olga L. Kartashova
Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
Email: labpersist@mail.ru
ORCID iD: 0000-0002-1487-7546
SPIN-code: 9315-1070
Scopus Author ID: 7003322797
ResearcherId: P-2645-2015
PhD, MD (Biology), Associate Professor, Leading Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, 460000, Orenburg, st. Pioneer, 11Lidiya P. Popova
Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
Email: labpersist@mail.ru
ORCID iD: 0000-0002-3766-6020
SPIN-code: 8577-1007
Scopus Author ID: 56946322300
ResearcherId: J-3339-2018
PhD, Senior Researcher, Laboratory of Persistence and Symbiosis of Microorganisms Russian Federation, 460000, Orenburg, st. Pioneer, 11
Michael D. Kuzmin
Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
Email: mdk-49@mail.ru
ORCID iD: 0000-0002-6349-6053
SPIN-code: 1895-4318
Doctor of Medical Sciences, Leading Researcher, Laboratory for the Study of Mechanisms of Formation of Human Microbial BiocenosesRussian Federation, 460000, Orenburg, st. Pioneer, 11
Viktor A. Gritsenko
Orenburg Federal Research Center of the Ural Branch of the Russian Academy of Sciences, Orenburg, Russia
Author for correspondence.
Email: vag59@mail.ru
ORCID iD: 0000-0002-2086-5170
SPIN-code: 7107-1300
Scopus Author ID: 7101892654
ResearcherId: O-9429-2015
PhD, MD (Medicine), Professor, Chief Research Associate, Laboratory of Persistence and Symbiosis of Microorganisms
Russian Federation, 460000, Orenburg, st. Pionerskaya, 11References
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