IMBALANCE IN PERIPHERAL BLOOD “POLARIZED” T-HELPER SUBSETS DURING MULTIPLY SCLEROSIS

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Abstract

Multiple sclerosis (MS) is the inflammatory disease of the central nervous system characterized by multifocal areas of demyelization and immune cell infiltration. In the current study, we analysed the frequencies and the phenotypes of “polarized” Th cell subsets in peripheral blood of multiple sclerosis patients (n=38, with low disease duration – 1 year (8 month; 1,5 years) and low EDSS – 1,0 (1,0; 1,5)) and healthy control group (n=48). We show that within CD45RA–CD3+CD4+ subset the frequencies of Th17 and follicular Th (Tfh) cells were signifi cantly higher in patients while the levels of Th1 cells were significantly lower in comparison to control group. Similarly, in central memory CD45RA–CD62L+ Th the relative number of Th1 cells was reduced while frequencies of Tfh subset were increased in MS patients if compared to control. Finally, MS patients showed increased frequency of Th17 and Tfh cells in CD45RA–CD62L– Th subset compared to control while Th1 were lower in patients group. It seems that Th17 cells infiltrating the nervous tissue could be responsible for neutrophil infiltration, while Tfh cells could take part in B-cell-mediated immune response nearby to site of infl ammation and may be accompanied by production of self-reactive antibodies as well as augmented neutrophilic activity. 

About the authors

I. V. Kudryavtsev

Institute of Experimental Medicine (FSBSI “IEM”);
N. N. Petrov National Medical Research Center of Oncology

Author for correspondence.
Email: igorek1981@yandex.ru

PhD, senior researcher, department of immunology,

senior researcher, department of oncoimmunology,

St. Petersburg

Russian Federation

A. G. Ilves

N. P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences
(IHB RAS)

Email: fake@neicon.ru

PhD, senior researcher, laboratory of neuroimmunology, 

St. Petersburg

Russian Federation

A. V. Il’chenko

Institute of Experimental Medicine (FSBSI “IEM”)

Email: fake@neicon.ru

research assistant, department of immunology,

St. Petersburg

Russian Federation

I. I. Krobinets

Russian Research Institute of Hematology and Transfusiology

Email: fake@neicon.ru

PhD, Senior Research Associate, 

St. Petersburg

Russian Federation

O. M. Novoselova

N. P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences
(IHB RAS)

Email: fake@neicon.ru

junior researcher, laboratory of neurorehabilitation, 

St. Petersburg

Russian Federation

K. S. Rubanik

N. P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences
(IHB RAS)

Email: fake@neicon.ru

junior researcher, laboratory of neurorehabilitation, 

St. Petersburg

Russian Federation

M. K. Serebryakova

Institute of Experimental Medicine (FSBSI “IEM”)

Email: fake@neicon.ru

researcher, department of immunology, 

St. Petersburg

Russian Federation

L. N. Prakhova

N. P. Bechtereva Institute of the Human Brain of the Russian Academy of Sciences
(IHB RAS)

Email: fake@neicon.ru

MD, Head of the laboratory of neurorehabilitation, 

St. Petersburg

Russian Federation

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Copyright (c) 2019 Kudryavtsev I.V., Ilves A.G., Il’chenko A.V., Krobinets I.I., Novoselova O.M., Rubanik K.S., Serebryakova M.K., Prakhova L.N.

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