Email this article FEATURES OF PROINFLAMMATORY ACTIVATION OF MACROPHAGES IN PATIENTS WITH RHEUMATOID ARTHRITIS AND SYSTEMIC LUPUS ERYTHEMATOSUS
- Authors: Bogatyreva A.I.1, Kiseleva D.G.1,2, Cherednichenko V.R.1, Markina Y.V.1, Kirichenko T.V.1
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Affiliations:
- Petrovsky National Research Centre of Surgery, Moscow, Russia
- Lomonosov Moscow State University, Moscow, Russia
- Section: Joint Immunology Forum 2024
- URL: https://rusimmun.ru/jour/article/view/16673
- DOI: https://doi.org/10.46235/1028-7221-16673-FOP
- ID: 16673
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Abstract
AbstractAutoimmune rheumatic diseases (ARDs) are chronic pathological conditions that arise from an abnormal immune response and are accompanied by systemic inflammation. The most common ARDs include rheumatoid arthritis (RA), systemic lupus erythematosus (SLE) and systemic sclerosis (SSc). The exact pathogenesis of ARDs remains unclear, but the complex influence of genetic, immunological and external environmental factors leads to the occurrence and further progression of ARDs. It has been shown that the cause of chronic inflammation may be pro-inflammatory activation of macrophages, in which an increase in the secretion of cytokines is observed. The aim of this study was to evaluate the inflammatory response of macrophages in patients with RA, SLE and SSc. Materials and methods. The study included 143 participants: 47 patients with RA, 45 patients with SLE, 34 patients with SSc and 17 people without ARDs and other chronic diseases. Isolation of a primary culture of monocytes was carried out by centrifugation in a ficoll gradient using magnetic separation from the whole blood of study participants. Lipopolysaccharide (LPS) was added to stimulate cells along the proinflammatory pathway. Cell cultivation was carried out for 24 hours. Determination of basal and LPS-stimulated secretion of IL-8 by macrophages was carried out in the culture fluid using an enzyme-linked immunosorbent assay (ELISA). Proinflammatory activation of macrophages was calculated as the ratio of LPS-stimulated and basal IL-8 secretion. Research results. Basal secretion of IL-8 by macrophages was statistically significantly higher in the groups of patients with RA and SSc compared with the SLE and control groups. LPS-stimulated secretion of IL-8 by macrophages in the SSc group had statistically higher values compared to the RA and SLE groups. Pro-inflammatory activation of macrophages was reduced in the group of patients with RA compared to patients with SLE and the control group, and was also statistically significantly lower in patients with SSc compared to the control group.
About the authors
Anastasia I. Bogatyreva
Petrovsky National Research Centre of Surgery, Moscow, Russia
Email: nastya.bogatyreva.96@mail.ru
ORCID iD: 0000-0002-1188-1945
Researcher, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 119435, Moscow, Abrikosovsky lane, 2Diana G. Kiseleva
Petrovsky National Research Centre of Surgery, Moscow, Russia;Lomonosov Moscow State University, Moscow, Russia
Email: trueit1292@gmail.com
Junior Researcher, Department of Biophysics, Faculty of Biology; Junior Researcher, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 119435, Moscow, Abrikosovsky lane, 2; 119234, Moscow, st. Kolmogorova, 1Vadim R. Cherednichenko
Petrovsky National Research Centre of Surgery, Moscow, Russia
Email: vadik2222111@gmail.com
Junior Researcher, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 119435, Moscow, Abrikosovsky lane, 2Yuliya V. Markina
Petrovsky National Research Centre of Surgery, Moscow, Russia
Email: yu.v.markina@gmail.com
ORCID iD: 0000-0002-3781-6340
Cand. Sci. (Med.), Senior Researcher, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 119435, Moscow, Abrikosovsky lane, 2Tatiana V. Kirichenko
Petrovsky National Research Centre of Surgery, Moscow, Russia
Author for correspondence.
Email: t-gorchakova@mail.ru
ORCID iD: 0000-0002-2899-9202
Cand. Sci. (Med.), Senior Researcher, Laboratory of Cellular and Molecular Pathology of the Cardiovascular System
Russian Federation, 119435, Moscow, Abrikosovsky lane, 2References
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СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).