BLOOD MYOKINE PROFILE IN ANTERIOR CRUCIATE LIGAMENT INJURY OF THE KNEE JOINT WITH THE PHENOMENON OF ARTHROGENIC MUSCLE INHIBITION

Abstract

Arthrogenic muscle inhibition is a poorly studied phenomenon that occurs in 30 to 50% of all injuries to the capsular-ligamentous apparatus of the knee joint. The study of the dynamics of myokine levels in the blood after an injury with the development of arthrogenic muscle inhibition is important for understanding the processes of restoration of the structures of the motor segment. Objective: to analyze the direction of changes in the content of myokines in the peripheral blood of patients with arthrogenic muscle inhibition (AMI) due to traumatic injury to the anterior cruciate ligament (ACL) of the knee joint. Materials and methods. The study included 58 men involved in aerobic sports (athletics, swimming, basketball) with ACL injury. To identify the AMI phenomenon, an electromyographic (EMG) study of the straight head of the quadriceps femoris muscle of the injured limb was performed on a 4-channel EMG/EP system "Viking Quest" (Nicolet, USA). Myokine levels in venous blood were determined using enzyme immunoassay test systems of domestic and foreign production: IL-6 (Vector-Best, Novosibirsk); concentration of transforming growth factor - β, decorin, myostatin (Cloud-Clone Corp. (China); VEGF (BioKhimMak CJSC). The control group consisted of 12 healthy men, average age 34.2±2.4 years. Statistical processing of the material was carried out using the Statistica. vers.10.0 package (StatSoft Inc., USA). Results. In patients with ACL injury and the presence of the AMT phenomenon, a significant decrease in the concentrations of IL-6, transforming growth factor β and proteoglycan - decorin in the blood and an increase in the concentrations of VEGF and MSTN, which, along with TGF-β, belong to the group of growth factors, were found in comparison with the indicators of patients with ACL injury but do not have the AMT phenomenon. Discussion: A decrease in the level of IL-6, decorin and VEGF is probably a consequence of forced hypokinesia in ACL injury, and the formation of AMT can also be a consequence of the increase in myostatin in the blood, which is a negative regulator of myogenesis. Conclusion: changes in the concentration of myokines in the peripheral blood during traumatic damage to the capsular-ligamentous apparatus of the knee joint clearly demonstrate the involvement of the muscle secretome in the processes of traumatic tissue damage and the formation of the phenomenon of arthrogenic muscle inhibition.