CSF-1/CSF1R SYSTEM AS PREDICTOR OF LIVE BIRTH AFTER INDUCED PREGNANCY

Abctract

The effectiveness of the IVF procedure is assessed not only by the indicators of the onset of clinical pregnancy, but also by the proportion of live births, since about a third of pregnancies end in termination, which is certainly determined by many factors. Colony-stimulating factors can be considered potential predictive markers of the induced pregnancy development and resulting live birth. To evaluate the predictive value of the CSF-1 system (serum CSF-1 concentration and carriage of CSF1R gene polymorphisms) in the setting of live birth after IVF-induced pregnancy in women with tuboperitoneal infertility. 88 patients undergoing IVF aged between 25 and 40 years were assigned to the following groups: Group I (n = 32): patients whose post-IVF pregnancies resulted in live births at Week 24 or later; and Group II (n = 52): patients whose pregnancies did not occur or resulted in spontaneous miscarriages. ELISA determined the CSF-1 levels twice: on pre-IVF menstrual cycle Days 3–4, and on post-embryo-transfer Day 15. Genotyping according to the CSF1R gene rs3216780 and rs38669350 polymorphic markers used a polymerase chain reaction with Sanger sequencing. CSF-1 levels grew to post-embryo-transfer Day 15 (3.9-fold in Group I and 1.8-fold in Group II); in the Group II pregnant patients, they were reliably higher than in the comparison group (p = 0.0017). Most women whose pregnancy resulted in live births carried the del/G rs3216780 and TG/CA rs386693509 genotypes of the CSF1R gene. Univariate analysis identified the following predictors for the completion of induced pregnancy by delivery - peripheral blood CSF-1 levels from 121.3 to 314.8 pg/mL at the preconceptional stage and from 963.3 to 1682.8 pg/mL - on day 15 after embryo transfer, as well as carriage of del/G rs3216780 genotypes and TG/CA rs386693509 gene CSF1R.V combination, these predictors were observed in 66.7% of women from group I and in 7.7% from group II. The CSF-1 system plays an important role in the realization of a woman's reproductive function and the identified prognostic indicators - the pre-conception and post-ET Day 15 serum CSF-1 concentrations and the carriage of the CSF1R gene del/G rs3216780 and TG/CA rs386693509 genotypes can be used as predictors of the completion of induced pregnancy by childbirth.