Email this article PHENOTYPING OF ANTIGEN-SPECIFIC CYTOTOXIC T-LYMPHOCYTES BY THE METHOD OF MULTICOLOR FLOW CYTOMETRY
- Authors: Kuznetsova M.S.1, Lopatnikova J.A.1, Sennikov S.V.1,2
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Affiliations:
- Research Institute of Fundamental and Clinical Immunology
- Novosibirsk State University
- Issue: Vol 22, No 2-2 (2019)
- Pages: 837-839
- Section: ORIGINAL ARTICLES
- Submitted: 16.06.2020
- Accepted: 16.06.2020
- Published: 15.07.2019
- URL: https://rusimmun.ru/jour/article/view/357
- DOI: https://doi.org/10.31857/S102872210006680-3
- ID: 357
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Abstract
Modern methods of multicolor flow cytometry in combination with MHC multimer technologies allow not only to identify and isolate cytotoxic T-lymphocyte (CTL) populations, but also to conduct phenotypic research on the level of differentiation, functional features of these cells, and even to investigate the distribution of memory T-cell subsets inside populations specific to particular epitopes of tumor antigens. In the present study, we assessed the level of differentiation and the content of memory T-cell subpopulations in cytotoxic T-lymphocyte populations specific for HER2/neu antigen epitopes (HER2) using the MHC-multimers antigen-specific CD8+ T-cell staining method and multicolor flow cytometry. The resulting HER2-specific T cells to a large extent (about 40‒50%) have the TSCM phenotype, a population capable of the most pronounced antitumor immune response due to a combination of effector properties with self-sustainability.
About the authors
M. S. Kuznetsova
Research Institute of Fundamental and Clinical Immunology
Author for correspondence.
Email: fake@neicon.ru
junior researcher of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationJ. A. Lopatnikova
Research Institute of Fundamental and Clinical Immunology
Email: fake@neicon.ru
PhD, senior researcher of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationS. V. Sennikov
Research Institute of Fundamental and Clinical Immunology;Novosibirsk State University
Email: sennikovsv@gmail.com
PhD, MD, professor, head of the Laboratory of Molecular Immunology,
Novosibirsk
Russian FederationReferences
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Supplementary files
СМИ зарегистрировано Федеральной службой по надзору в сфере связи, информационных технологий и массовых коммуникаций (Роскомнадзор).