Influence of time of cultivation of Staphylococcus aureus on the production of cytokine-like substances that detected by ELISA

The paper presents the results of the study of supernatants 24 and 48 hours cultures of various strains of Staphylococcus aureus by the method of enzyme immunoassay (ELISA) by means of test-systems for determining the levels of human cytokines INF-α, INF-γ, IL-1Ra, IL-1β, IL-4, IL-6, IL-8, IL-10, IL-17, TNF-α (“Cytokine”, St. Petersburg). It has been established that a number of strains of staphylococci are able to secrete various cytokine-like substances (CLSs), determined by test systems for ELISA, in the incubation medium. The highest rates were established for CLSs, “identical” to human cytokines INF-γ, INF-α, IL-4 and IL-1Ra. The remaining CLSs were determined in minimum concentrations. At the same time, the level of bacterial CLSs that was detected in high concentrations signifi cantly increased signifi - cantly with increasing incubation time of S. aureus from 24 to 48 hours.