Email this article DETERMINATION OF IL-10 AND IL-17 IN CELL CULTURE SUPERNATANTS DURING THE EXPANSION OF GAMMA DELTA T LYMPHOCYTES FROM THE BLOOD OF PATIENTS WITH REACTIVE ERYTHEMA
- Authors: Sorokina E.V.1,2, Kalinichenko E.O.1, Bisheva I.1, Stolpnikova V.N.1, Skhodova S.A.1
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Affiliations:
- I.I. Mechnikov Research Institute of Vaccines and Serums, Russian Academy of Medical Sciences, Moscow
- Federal state budgetary educational institution of additional professional education Institute of advanced training of Federal medical and biological Agency, Moscow
- Section: ORIGINAL ARTICLES
- Submitted: 12.06.2025
- Accepted: 24.07.2025
- URL: https://rusimmun.ru/jour/article/view/17281
- DOI: https://doi.org/10.46235/1028-7221-17281-DOI
- ID: 17281
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Abstract
Abstract
Reactive erythemas are a group of dermatoses with complex immunogenesis involving subpopulations of γδ T cells. Despite the important role of IL-17, its overproduction, including by γδ T cells, is capable of inducing autoinflammation. On the contrary, IL-10 plays a key role in the control of innate immune responses by suppressing the function of monocytes/macrophages. Goal. Expansion of γδ T cells by in vitro cultivation of lymphocytes from the blood of patients with erythema in the presence of zoledronate, IL-2 and IL-15 and determination of the content of IL-17, IL-10 in supernatants. Materials and methods. Peripheral blood mononuclear cells were isolated from 8 patients with reactive erythema (5 patients with erythema multiforme, 1 patient with ring–shaped centrifugal erythema, 2 patients with erythema migrans, an early form of Lyme disease) and two healthy donors aged 23 to 70 years. A modified van Ackeretal method (2016) was used to culture γδ T lymphocytes. Cytokine concentrations were determined by solid-phase ELISA using Human IL-17 ELISA Kit (ABclonal BiotechnologyCo., China) and Interleukin-10-ELISA-BEST (Vector Best, Russia).
Results and conclusion. In patients with reactive erythema, 3.1% to 62.8% of cells with expression of γδ TCR were obtained by 7 days of cultivation with zoledronate, IL-2 and IL-15. The minimum percentage of these cells was observed in blood cell culture from patients with continuously recurrent erythema multiforme and ring-shaped centrifugal erythema. In the examined patients, high levels of induced IL-17 production may indicate the presence of an autoimmune component in the pathogenesis of the disease and reflect the severity of the course. The effectiveness of therapy correlated with the ability of blood cells to synthesize and release IL-10. It has been demonstrated that high levels of IL-10 production are associated with significant clinical improvement and long-term clinical remission. On the contrary, with a low therapeutic effect, low values of spontaneous and induced IL-10 production were revealed. The determination of these analytes (IL-17, Il-10) in PBMC culture in patients with the studied pathologies in the future will improve the assessment of the effectiveness of therapy and predict the course of the disease.
About the authors
Ekaterina Vyacheslavovna Sorokina
I.I. Mechnikov Research Institute of Vaccines and Serums, Russian Academy of Medical Sciences, Moscow;Federal state budgetary educational institution of additional professional education Institute of advanced training of Federal medical and biological Agency, Moscow
Author for correspondence.
Email: sorokina-cathrine@yandex.ru
ORCID iD: 0000-0002-1188-6578
SPIN-code: 1052-6474
Doctor of Medical Sciences, Head of the Laboratory of Mechanisms of Regulation of Immunity at the I.I. Mechnikov Research Institute of Vaccines and Serums, Professor.
Russian Federation, 105064, Russia, Moscow, Maly Kazenniy lane, 5a.Eugen Olegovich Kalinichenko
I.I. Mechnikov Research Institute of Vaccines and Serums, Russian Academy of Medical Sciences, Moscow
Email: gladius.domini@gmail.com
Candidate of Medical Sciences, researcher at the Laboratory of mechanisms of regulation of Immunity.
Russian FederationIrina Bisheva
I.I. Mechnikov Research Institute of Vaccines and Serums, Russian Academy of Medical Sciences, Moscow
Email: ibisheva@yandex.ru
ORCID iD: 0000-0002-8143-7356
SPIN-code: 9846-1006
Researcher, Laboratory of mechanisms of regulation of immunity.
Russian Federation, 105064, Russia, Moscow, Maly Kazenny pereulok, 5a.Vera Nikolaevna Stolpnikova
I.I. Mechnikov Research Institute of Vaccines and Serums, Russian Academy of Medical Sciences, Moscow
Email: stolpnikova@yandex.ru
Candidate of Biological Sciences, leading researcher at the Laboratory of mechanisms of regulation of Immunity.
Russian Federation, 105064, Russia, Moscow, Maly Kazenny lane, 5a.Svetlana Anatolievna Skhodova
I.I. Mechnikov Research Institute of Vaccines and Serums, Russian Academy of Medical Sciences, Moscow
Email: skhodova2009@yandex.ru
ORCID iD: 0000-0002-2342-9307
Candidate of Biological Sciences, leading researcher at the Laboratory of mechanisms of regulation of Immunity.
Russian Federation, 105064, Russia, Moscow, Maly Kazenny lane, 5a.References
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