CATALASE AND SOD ACTIVITY OF SERUM IMMUNOGLOBULIN G IN COLORECTAL CANCER



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Abstract

Abstract

The study of antibodies with catalytic properties, in particular IgG with oxidoreductase activities in cancer pathology is a relatively new direction in oncology, with very few publications. The aim of the present work is to investigate the catalase and superoxide dismutase (SOD) activities of IgG from patients with colorectal cancer (CRC) depending on the clinical and morphologic parameters of the disease. Materials and Methods. Blood serum from 20 patients with colorectal cancer (T3-4N0-2M0) with highly and low-differentiated tumors and 18 conditionally healthy donors was used in the study. The groups were comparable by sex and age. Samples of IgG were received from serum by affine chromatographic method on column with Protein-G-Sepharose on the chromatograph AKTA pure (GE, USA). Dialysis of antibodies was performed against 20 mM phosphate buffer, pH=7.0. IgG concentration was determined by spectrophotometric method on a Varioskan LUX multifunctional reader (Thermo Seinctific, USA). Electrophoretic analysis of the obtained samples was carried out according to the Lemmli method in gradient 4-18% PAGE. Visualization of the gels was performed using an iBright Imaging Systems FL1500 (Thermo Scientific, USA). Catalase and SOD activities of the antibody samples were spectrophotometrically determined on a Varian Cary 60 UV-Vis spectrophotometer (Agilent, USA). Statistical processing of the data was carried out in Statistica 12.0 program. Results. In the presented work, it is shown for the first time that IgG of CRC patients are able to catalyze catalase and SOD reactions and that this activity is an intrinsic property of the antibodies. It was revealed that both studied IgG activities were significantly increased in patients compared to healthy individuals. It is shown that IgG catalase activity in CRC patients with the second stage of tumor process is 1.8 times higher (p=0.015) in comparison with the similar activity in CRC patients with the third stage of tumor process. The IgG catalase activity in patients with low-differentiated CRC is significantly decreased by 2 times (p=0.045) in comparison with patients with highly differentiated CRC. On the contrary, IgG SOD activity in patients with highly differentiated CRC is 1.7 times lower (p=0,021) in comparison with patients with low-differentiated CRC. Thus, the studied oxidoreductase activities of abzymes in patients with CRC obviously make a significant contribution to the pathogenesis of CRC and can be used for prognosis of the disease development.

About the authors

Liudmila P. Smirnova

Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

Email: lpsmirnova@yandex.ru
ORCID iD: 0000-0003-0083-9124
SPIN-code: 2351-1097
Scopus Author ID: 7102578775
ResearcherId: C-9026-2012
https://www.tnimc.ru/kontakty/personalii/nii-psikhicheskogo-zdorovya/smirnova-lyudmila-pavlovna/

MD, PhD, Senior Researcher Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

Russian Federation, 634014 Tomsk, RF, Aleutskaja, 4, Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences

Valeria S. Voronina

Siberian State Medical University, Tomsk, Russia

Email: herman.virtt@gmail.com
ORCID iD: 0009-0009-4665-1961

student of the Siberian State Medical University

Russian Federation, 634050, г. Томск, ул. Московский тракт, 2 ст20

Polina A. Antipina

Siberian State Medical University, Tomsk, Russia

Email: pol.antipina2015@yandex.ru

student of the Siberian State Medical University

Russian Federation, 634050, г. Томск, ул. Московский тракт, 2 ст20

Daria V. Kazantseva

Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

Email: dashk1745@mail.ru
ORCID iD: 0000-0001-8050-269X
Scopus Author ID: 57221819468
ResearcherId: GLU-6511-2022

graduate student of the Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Junior Researcher Laboratory of Molecular Genetics and Biochemistry, Mental Health Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

Russian Federation, 634014 Томск, Россия, Алеутская 4, НИИ психического здоровья ТНИМЦ

Dmitry N. Kostromitsky

Cancer Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

Email: d.n.kostromitsky@gmail.com
ORCID iD: 0000-0001-5691-2349

MD, PhD, Senior Researcher Abdominal Oncology Department, Cancer Research Institute, Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia

Russian Federation, 634009, г. Томск, пер. Кооперативный, 5

Natalia V. Yunusova

Cancer Research Institute of Tomsk National Research Medical Center of the Russian Academy of Sciences, Tomsk, Russia;
Siberian State Medical University, Tomsk, Russia

Author for correspondence.
Email: BochkarevaNV@oncology.tomsk.ru
ORCID iD: 0000-0003-4595-4177

MD, Chief Researcher, Laboratory of Tumor Biochemistry, Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Russian Federation, 634009, г. Томск, пер. Кооперативный, 5

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Copyright (c) Smirnova L.P., Voronina V.S., Antipina P.A., Kazantseva D.V., Kostromitsky D.N., Yunusova N.V.

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