Vol 20, No 3 (2017)

The decrease of the relative content of CD3+ and absolute content of CD4+ lymphocytes were observed among the personsexposed to the chronic irradiation with elevated levels of chromosomal aberrations in peripheral blood lymphocytes.

The purpose of the study is the definition of characteristics of the response of the immune system of patients with lung tuberculoma in the active phase and phase subsides / moderate activity for the establishment of the laboratory criteria for preoperative evaluation of educational activity. The study was performed with the participation of 160 people, We studied the absorption and functional metabolic activity of neutrophils and monocytes, population composition of cells, expression of adhesion molecules and markers of cell activity, the concentration of cytokines. Determine CD11b expression tests and HLA-DR on monocytes, CD11b - on neutrophils, evaluations populations CD3+HLA-DR+ and CD3⁺CD4⁺CD25^high can be recommended for preoperative assessment of activity tuberculoma lung.

Significant local antimicrobial factors of the intestinal biotope and cytokines associated with the variant of the course of reactive arthritis (ReA): IL-17, IL-6, INF-γ, lysozyme, CRB have been determined, which allows them to be used as markers for predicting the course and the outcome of ReA.

In this study, for the first time, shows the role of lipoic acid in the correction of glucose, glycated hemoglobin, insulin, pro - and anti-inflammatory cytokines in the blood of rats in the formation of alloxan diabetes.

In experiments in vitro and in vivo it was shown that a synthetic peptide of the active site of GM-CSF -ZP2 (chemical formula: THR NLE NLE ALA SER HIS TYR LYS GLN HIS CYS PRO) stimulates the activity of immune cells (lymphocytes, stem cells, neutrophils, monocytes), inhibits the growth, reproduction and biofilm formation of gram-positive and gram-negative bacteria and has antiviral activity against DNA and RNA viruses and activates the repair of damaged tissues of the organism at the wound process. The presence at this synthetic peptide of such a wide range of immunological effects (immunostimulatory, antibacterial, antiviral, and repair activity) determines the possibility of its use as the basis for creation of new effective medicinal and cosmetic preparations with combined properties that were implemented in the creation of cosmetics ACEGRAM.

The effect of iron ions on biochemical blood indices was evaluated to study the effectiveness of probiotic preparations on the basis of bacteria of the genus Bacillus in the experimental intoxication of laboratory animals with iron. It has been established that a single oral administration of ferrous sulfate leads to an increase in the studied biochemical parameters in experimental groups with the addition of metal.

At athletes with mainly aerobic power supply of motive activity and datum level of the relative index of an entropy of a leukocytic blood count the essential fluctuations of indexes of an immunogram in an annual cycle of preparation caused by impact of a rhythm of exercise stresses on process of adaptation of an organism to seasonal changes of conditions of the environment are shown.

We examined 266 patients 15-17 years old with menstrual disorders. Prevalence of oligomenorrhea in the Eastern zone, which is unfavorable environment. The levels of relative and absolute СD3⁺, СD4⁺ and СD8⁺ contents, the absolute amount of СD19⁺, FP, IgA, IgG, elevations of cortisol, testosterone, FSH, LH, and 17-OH-progesterone have been decreased. Indicators show the impact of the anthropogenic load on gynecological morbidity.

Children with reactive arthritis (n=26) of 2-17 years old were examinated. Antinuclear antibodies were determined by indirect immunofluorescence assay, antibodies to extractable nuclear antigens were defined by ELISA. It was found that 12 patients (46 %) with reactive arthritis had diagnostic titers of antinuclear antibodies detected by indirect immunofluorescence assay. Homogeneous and speckled fluorescence patterns were observed in patients with reactive arthritis. The prevalence of antibodies to extractable nuclear antigens in children with reactive arthritis was low (8 %).

Women with tubal peritoneal infertility with an effective IVF had concentrations of IL-1a, IL-1ra in serum lower that women ineffective IVF. Genotype T/T gene IL-1a rs1800587 5'UTR area of the gene is associated with approach of pregnancy as a result of IVF (OR = 85,3, 95 %CI: 10,6-687, p<0,05).

The study of the distribution frequency of polymorphic variants of genes TNF - α and IL-10 in miscarriage, the first trimester. It is found that the carriers of allele A of the gene of TNF-α risk of pregnancy in urogenital infections is higher and the GG genotype is protective, which contributes to a more favorable course of pregnancy.

A comparative analysis of the secretory products of neutrophils in the reproductive tract of women with chronic inflammatory diseases caused by opportunistic microorganisms. Shows the different levels of the secretory peptides depending on the composition of microbial associations. Studied indicators of the level of immunoglobulins in the cervical secretion when combined PVI with opportunistic microorganisms.

The study presents the results of the study of the most significant cytokines of cervical mucus in women and the ejaculate of 38 married couples with a papillomavirus infection. Changes in the levels of indicators are as one-sided, expressed in an increase in the number of IL-2, IL-10, sIgA, and versatile, characterized by a decrease in IFN-γ in women, with its normal level in men.

Method of assessment of high risk of frequent cases of SARS by data from immunoepidemiological cross-sectional study are proposed. The data of the immunological examination of the workers in the chemical enterprise who had contact with a production factor were used to test it. The results are discussed.

In preterm delivery both in the 1and in the 3 trimesters of pregnancy there was an elevated level of ef-fectory memory cells in the subset of cytotoxic T lymphocytes. Thus, the preterm delivery was associated with the enhancement of CD8+ lymphocytes differentiation towards terminal stages.

Screening studies among 232 patients with chronic viral hepatitis B and C revealed systemic (organ-specific) autoantibodies in 43.8 % and 30.2 % of cases, respectively. When studying the characteristics of immune response of patients it was established that in case of chronic viral hepatitis B pathogenetic bases of development of autoimmune disorders of a systemic nature are the stimulation of production of antibodies by B lymphocytes, and a decrease in elimination of the pathogen, with a weakly expressed antiviral response. In chronic viral hepatitis C, autoimmune disorders of a systemic nature develop against a background of a deep T-cell immunodeficiency state and an inadequate antiviral response. And also with systemic autoantigenemia, along with high indicators of circulating immune complexes, which indicate activation of antiviral protection, there is a deficiency of phagocytic activity of neutrophils, which further aggravates the immunopathological process.

The decrease of the leiomyoma size under the influence of ulipristal acetate therapy took place in women with initially high level of endometrial TGFpi⁺ macrophages and with elevation of TGFpi⁺ and CD163+ macrophages after treatment. Lack of the treatment effect was associated with diminishment of M2 macrophages and increases the amount of IL-12+ macrophages.

Aim: to assess clinical value of antibodies to Herpes Simplex Virus (HSV) in outpatients with head and back pain. Cross sectional study included 45 female outpatients aged from 40 to 60. Two or more HSVinfec-tion exacerbations per year were registered in 25 persons (Group1) while 20 women had no exacerbation during previous year (Group 2). General practitioner and neurologist examination have been performed accompanied by IEA IgG anti-HSV type 1,2 antibodies assessment. Results: Group 1 showed 5-fold higher IgG anti-HSV antibodies level and post-herpetic neuralgia frequency then Group 2. High IgG anti-HSV antibodies level was associated with increased odds of HSV infection exacerbation (OR = 1.8; 95 % СШ 1.2-2.6).

The research presents the results of evaluation of therapeutic effect on a new drug composition, containing the Acegram and Silativit-gel on the model of chronic periodontitis in rats. Objective evidence of the efficacy of the analyzed drug composition was obtained.

Aim of the study: to investigate the effect of erythropoietin (EPO) on the parameters of cellular immunity in preterm infants (PI) of early preterm birth (EPB). The study was performed on 15 full-term infants and 25 PI from EPB (gestational age 28 weeks - 30 weeks and 6 days). EPO was applied 6 days of life at a dose of 200 IU/kg every 48 h. Number of subpopulations of leukocytes in blood was determined by flow cytometry with antibodies of system “CytoDiff”. It was found that PI from EPB at 8 and 18 days of life reduced the representation in blood of B-lymphocytes, T-helper cells, T - and NK-cells; on the 8th day of life increases the number of monocytes and their precursors decreases the number of eosinophils; 18 day - reduced the number of monocytes, mature neutrophils, increases the number of mast cells, precursors of B-and T-lymphocytes. The use of EPO leads on the 8 days of life to increase in blood the total number of lymphocytes due to T - and NK-cells, the number of immature granulocytes; 18 day - to increase the total number of lymphocytes at the expense of B-lymphocytes, T-helper cells, T - and NK-cells, increasing the number of mature neutrophils, immature granulocytes, the precursors of monocytes.

The aim of this work is to investigate the effect of transdermal film (TDF) with erythropoietin (EPO) on the parameters of innate and adaptive immunity in experimental thermal injury (TI). The experiment was carried out on 70 white nonlinear Mature rats, which were divided into 3 groups: 1 (n=10) - intact control, 2 (n=30) -TI, 3 (n=30) -TI with the imposition of TDF with EPO. TI IIIA degree with an area of 3.5 % was modeling by immersion of the site interscapular region of the animal in clean water with a temperature of 98-99°С for 12 s. the Study was performed at 3rd, 8th and 14th days TI. The number of neutrophils in the blood was determined by hematological analyzer “Medonic - M 16” (Sweden). The absorption ability of neutrophils blood was examined using of latex particles, the oxygen-dependent metabolism in spontaneous and induced NST-test. With the help of a flow cytometer “Navios” in the blood determined the number of CD3+ and CD45RA+ lymphocytes. The death of lymphocytes was assessed by staining cells with fluorochrome conjugated with annexin V (Annexin-5-FITC) and 7-aminoantipyrine D (7-AAD). It was found that experimental TI in the blood increases the number of neutrophils, their phagocytic and NST-reducing ability, reduced number of CD3+ and CD45RA+ lymphocytes at 5th, 8th and 14th days, increases in blood lymphocyte count with evidence of apoptosis and/or necrosis at 3rd and 8th days. The use of EPO in the composition of TPF in the TI did not accompanied by a change in the number of neutrophils in the blood at 3rd, 8th and 14th days, leads to a decrease in phagocytosis activity at day 14th, the intensity of phagocytosis - on the 8th day, activity and the intensity of spontaneous NST-test at day 14th, the induced NST-test - 3rd, 8th and 14th days; increase of CD3+ lymphocytes in the blood on the 8th day, the reduction in the number of blood lymphocytes with signs of apoptosis and/or necrosis at 14th day.

It has been established that the level of IL-6 in patients with subcompensated CP (class B) was significantly higher than in the compensated stage (p <0.001), and also had significant differences in patients with subcompensated and decompensated CP (p <0.001). The concentrations of VEGF and G-CSF significantly increased at the stage of severe cirrhosis (p <0.001 and p = 0.04, respectively). Proinflammatory cytokine TNF-α and anti-inflammatory IL-4 significantly increased during transition to the subcompensated stage of the disease (р=0.01 and р=0.04, respectively). There are no reliable differences between allelic variations of polymorphic gene loci TNF (G4682A), IL-6 (C174G) and VEGFA (G-634C), associated with liver cirrhosis severity levels, detected by us.

The aim of this work was to investigate an antimicrobial properties of platelets of domestic horse (Equus ferus caballus) and isolate an antimicrobial components. Using a liquid chromatography technique (RP-HPLC) ten fractions were collected from platelet's acid extract. Among these a four fractions demonstrated antimicrobial activity against S.aureus.

Infant rats born from Wistar females who were exposed to secondhand smoke throughout pregnancy showed the decreased parameters of sensomotor and somatic growth, disorders of structural and functional activity and low number of thymocytes and splenocytes.

Growth and viability in different culture media after thawing of hybridoma BAP3 cells that produces monoclonal antibodies against pregnancy-specific b1-glycoprotein (PSG) were studied in vitro. We show that cultivation in [DMEM+12 % FCS] leads to maximal cell growth (1,8-fold in two days) with retention of viability of more than 70 %. There were 1,1-1,2-fold growth and viability of 55-59 % in [DCCM-1]-based media and 1,6-fold growth in two days in [RPMI-1640+12 % FCS]. Therefore, application of [DMEM+12 % FCS] medium seems to be the most appropriate for cultivation of hybridoma BAP3 cells after their thawing from cryopreservation.

To assess the functional activity of monocytes in preterm infants of different gestational age (22-27.6 weeks and 28-31 weeks) in the dynamics of the neonatal period, cord and peripheral blood were examined. All children with an ENMT at birth had a decreased willingness of effector cells to participate in intercellular interactions (decrease in the number of CD14+ CB 282+ and CD14+ CD157+ monocytes), in infants of gestational age 22-27 weeks - to antigen presentation of the pathogen (decrease in HLA receptor expression -DR+). At the end of the neonatal period, the functional activity of monocytes is restored, the number of CD14+ HLA-DR+ cells exceeds those of full-term children.

Comparative evaluation of character of the expression of TLR 9, CD11b and CD14 receptors monocytes whole blood under the influence of Derinat and synthetic TLR 9 ligand in healthy individuals and in acute infectious diseases of viral and bacterial origin has been studied. It has been shown that the unidirectional effects of study drugs relate changes in the number of monocytes only in groups with infectious process. Differently directed effects identified in relation to expression of molecules QD11b monocytes in all groups.

Against the background of treatment of IHCS of bronchial asthma, clinical remission, perhaps due to a change in the deviation of the immune response to the Th1 immune response.

The aim of this study was to detect sIgE to isoallergens of birch pollen and to evaluate a contribution of some homologous food allergens to the development of sensibility in AR patients. There were 21 blood sera of AR patients and 20 blood sera of healthy persons without allergy symptoms. The total IgE levels and sIgE to allergens were measured by the ImmunoCAP method (Phadia, Sweden), as well as by the ELISA kits (Alkorbio, Russia). We detected high total IgE levels, sIgE to allergens of birch pollen, apple, celery, as well as to recombinant allergens of birch Bet v 1, Bet v 2 and soybean - Gly m 4 in AR patients. Correlation analysis showed the direct valid dependence between the sIgE levels to birch isoallergen Bet v 1 and soy isoallergen Gly m 4 (r=0,84; p<0,05). There was also the direct valid correlation between the sIgE levels to birch isoallergen Bet v 1 and allergens of apple and celery (r=0,67 and 0,57, accordingly). Our finding permits assert that cross-reactivity can be important in exacerbation of clinical symptoms in AR patients including progress of oral allergy syndrome.

The phenomenon of detection of hemophagocytic macrophages in newborns with multiple organ dysfunction syndrome (MODS) was studied. It has been shown that detection of hemophagocytic macrophages in peripheral blood takes place in newborns with severe manifestations of MODS and is a prog-nostically unfavorable sign.

Clinical experience showed that use of the probiotic medicine “Sporobakterin” promotes not only the fissile elimination of ions of lead from an organism to 58,9 % (from an integument to 67,9 % muscular tissue to 47,1 % and a bone tissue to 61,5 %), but also prevents development of degenerative changes of internals that in our opinion has direct dependence. On by it application of Sporobakterin at treatment of intoxication lead interferes with development of inflammatory process in an organism what morphological, hematological and biochemical indexes of blood of the experimental animals testify to, and also have the stimulating effect of factors of nonspecific resistance of an organism.

The article present the results of a study of the diagnostic efficacy of various SIR indicators (IL-6, IL-8, IL-10, TNFa, CRP), duration of dialysis before renal transplantation, post-allotransplantation period and HLA mismatch score (the number of mismatches from 6 antigens HLA loci A, B, DR) with respect to the development of chronic renal allograft dysfunction (CRAD). It was shown that the level of proinflamma-tory cytokine TNFa is a promising marker for the prognosis of the CRAD development.

A study of immune regulation in children living in the region of industrial pollution by aluminum and other metals showed a decrease in phagocytic activity, expression of CD95 T-cell receptors, increased production of leukotrienes C4/D4/E4 and specific IgG to aluminum.

The study evaluated the state of innate immunity in young women with abdominal obesity and overweight. In women with isolated abdominal obesity the activation of neutrophil function was observed. During the progression of obesity in young women the decreasing of parameters of phagocytosis was revealed. It was associated with increased volume of abdominal fat tissue and disorders of lipid and carbohydrate metabolism.

The functional condition of skin directly coreesponds to its appearance. The cooMinated woA of the main cells of skin defines ability to support a homeostasis of skin and to react to influence of external and internal environment. Age dynamics of separate “immunocytes of skin” is traced (T, B- lymphocytes, monocytes/macrophages, co^ulent cages). External characteristics of skin have distinct collation communications with the studied parameter. Apparently, there is a possibility of influence on appearance of skin by impact on resident immunocompetent cells of skin.

Character of immune infringements essentially depends on pathogenetic variant of the diabetic foot syndrome and its severity degree. Results confirm pathogenetic heterogeneity of clinical forms of complicated diabetic foot syndrome and demand the differentiated approaches to treatment.

In the cascade of the phenomena, leading to development of deregulation pathology, the important place is allocated to change of activity of brain structures, including reason of their immune damage, stimulation or blockade. Injection of antinigra antibodies to black substance is followed by decrease in efficiency of mechanisms of organ resistance in system of external breath that is shown in deterioration in biophysical properties of surfactant and reduction of number and activity of phagocyting alveolar macrophages.

The aim of our study was to evaluate clinical and immunology efficiency of bovgualuronidaza azok-simer (“Longidaza”) in women with active nonspecific chronic endometritis and uterine fibroids treat-men. Bovgualuronidaza azoksimer inclusion in women with chronic endometritis of nonspecific etiology and uterine fibroids complex treatment increases clinical efficiency and improves indicators of innate immunity.

This article discusses the possibility of predicting post-transplant complications. The aim of our work is to evaluate the association of pre-operative level 21 of the cytokine in the blood plasma and during the post-transplant period. The analysis was first the differences in the initial level of biomarkers in patients without complications and with complications after transplantation. Our results confirmed that the screening of cytokines prior to transplantation may be used to predict the subsequent development of complications.

Chemotaxis and other factors of innate immunity are involved in metastasis (such as TLRs), it is advisable to study the effect of both TLRs and chemokines on the chemotaxis of tumor cells. The aim. Study and compare the chemotaxis of mononuclear cells and tumor cells towards TLRs ligands and CXCL12. Materials and methods. Chemoattractants were synthetic TLR ligands (DNA_lig, RNA_lig) and CXCL12. The study of cell migration was carried out in dynamics with the help of the Boyden chamber. Results. ^emotaxis of MNC from healthy donors exceeds the chemotaxis of tumor cells by 4 times and significantly differs by 1,6 times from spontaneous migration. Directional chemotaxis of MNCs of healthy donors in 1,7 and 1,5 times greater than the migration of MNCs of healthy donors after 60 minutes and 24 hours, respectively, relative to migration to DNA_lig and RNA_lig. Conclusion. From the results obtained, it can be seen that CXCL12-induced chemotaxis is higher in MNCs of healthy donors than in tumor cells. Similarly, chemotaxis relative to DNA_lig and RNA_lig is significantly lower than in the control. Thus, it can be said that TLRs ligands may inhibit migratory activity.

Simultaneous performance of laparoscopic splenectomy with elective caesarean section in pregnant women with severe refractory course of immune thrombocytopenia is a safe method, due to the low invasiveness, the expected lower blood loss and a fairly rapid postoperative recovery than is possible with an open access approach.

The level of B-lymphocytes, the major classes of Ig, circulating immune complexes(CIC) in blood of patients with aseptic necrosis of the femoral head (ANFH) before and after(in a year) total joint replacement surgery has been investigated. A significant reduction of initially high levels of IgG, CIC and b-lymphocytes after joint replacement is determined. The levels of IgG in the blood of patients with ANFH are considered as markers of the effective treatment.

The clinical and immunological examination of children with reactive arthritis (ReA) and discriminant analysis allowed to establish significant serum factors associated with the clinical variant of ReA (IL-17, IL-6, INF-γ, lysozyme, CRP), which indicates a possible Use them as markers for predicting the clinical course of ReA and the outcome of arthritis.

With the purpose of revealing the features of the innate immunity of premature infants with severe hypoxic-ischemic injury of the central nervous system, depending on the gestational age, a survey of deeply preterm infants born at the time of very early and early premature births was conducted. It was established that in all premature infants with severe hypoxic-ischemic CNS lesions, the level of expression of adhesion molecules on monocytes (CD14+CD11b+) and an increase in the level of IFN-γ and IL-8 at birth was observed at birth, regardless of the gestational age. The peculiarity of the immune response of children born in the term of early premature births is a decrease in the relative number of activated monocytes of CD14+CD64+ and CD14+HLA-DR, which indicates a disruption in the relationship between the cells of congenital and adaptive immunity and may be the cause of bacterial complications and a prolonged course of inflammatory Process.

It was established that acute stress leads to a decrease in the indices of luminol-dependent chemiluminescence in rats with zymosan-induced peritonitis, and administration of nadolol under stress conditions cancels this effect.

The influence of reproductive hormones on phenotypic characteristics, cytokine production and the level of lytic activity of natural killer (NK) cells was studied. The role of miRNAs in the hormonal mechanisms of formation of regulatory and cytotoxic NK cells subpopulations during pregnancy was investigated. The new physiological mechanisms of endocrine control of mother’s immune system NK cells that can be implemented in different periods of pregnancy were identified as a result of this work.

The aim of this study was to study the effect of the injectable form of platelet rich autoplasma on immunological indices of the oral cavity to fixed prostheses patients. The number and viability of leukocytes and phagocytic function of neutrophils of oral fluid to fixed prostheses patients was significantly lower than to patients without dentures. However, after the injection of platelet rich autoplasma indicators of cellular immunity to patients with fixed prostheses approached that of patients without dentures, which indicates a positive immunological effects of injection of platelet rich autoplasma.

The purpose of the study was the choice of composition, development of technology and the study of immunomodelling activity of suppositories, containing interferon alpha in combination acides nicotnic and succinic. When choosing the composition hydrophilic diphilic and lipophilic bases were examined. It was established that the dosage forms prepared with the use of polyethylene glycols provided a uniform plank mass. Taking into account the process of liberation in the conditions of dissolution temperature as well as spectrophotometric determination, the technology of the novel form of the drug form - suppositories with interferon alpha in combination with antioxidants.